2014
DOI: 10.1007/s12600-014-0445-y
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Development of an immunocapture–reverse transcription–polymerase chain reaction (IC-RT-PCR) using modified viral RNA release protocol for the detection of Grapevine leafroll-associated virus 3 (GLRaV-3)

Abstract: Grapevine leafroll-associated virus 3(GLRaV-3) is the most destructive virus causing leaf roll disease in grapevine. ELISA has been widely used to screen the propagating materials for indexing of this virus at nursery stage. But the uneven distribution of GLRaV-3 in vines, its confinement to phloem tissues and impact of seasonal influences on its concentration limit the scope of ELISA. RT-PCR (reverse transcription-polymerase chain reaction), is a more sensitive technique, but not feasible for large scale scre… Show more

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Cited by 15 publications
(5 citation statements)
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“…Planting pathogen free crop propagules is of paramount importance in grapevine for increasing the productive life of vineyards (Kumar et al, 2015). Traditional detection techniques like ELISA, PCR and their variants are employed for indexing of a few selected viruses of grapevine while certifying the planting material for commercial planting.…”
Section: Introductionmentioning
confidence: 99%
“…Planting pathogen free crop propagules is of paramount importance in grapevine for increasing the productive life of vineyards (Kumar et al, 2015). Traditional detection techniques like ELISA, PCR and their variants are employed for indexing of a few selected viruses of grapevine while certifying the planting material for commercial planting.…”
Section: Introductionmentioning
confidence: 99%
“…Similarly, in 2011, surveys were conducted in the grapevine orchards of Himachal Pradesh which recorded the presence of mixed infections of GLRaV-1 and GLRaV-3 in 29 per cent of the analyzed leaf samples through RT-PCR (Kumar et al, 2013). Subsequently, the presence of GLRaV-3 in five grapevine cultivars was also identified based on an Immunocapture (IC) RT-PCR assay (Kumar et al, 2015). Cost-effective, robust and efficient polyclonal antiserum was developed at ICAR-Indian Agricultural Research Institute, New Delhi using in vitro expressed CP of GLRaV-3 isolated from grapevine cv.…”
Section: Grapevine Leafroll-associated Virus -3 (Glrav-3)mentioning
confidence: 99%
“…Detection is the foremost step in devising disease management strategies for plant pathogens. In India, immuno-based diagnostics have been developed for three species of grapevine viruses viz., GLRaV-1 (Kumar et al, 2012b;Kumar et al, 2018), GLRaV-3 (Kumar et al, 2015;2018;Sidharthan et al, 2020Sidharthan et al, , 2022 and GLRaV-4 (Rai et al, 2018). Although 86 viruses are known to infect grapevine worldwide (Martelli, 2018;Fuchs, 2020), the preparedness for diagnosis of grapevine viruses exists hardly for a few viruses in India.…”
Section: Introductionmentioning
confidence: 99%
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“…IC-PCR is used for routine indexing of BSV worldwide [307,308]. It has also been useful for PRSV detection in leaf extracts of papaya and various cucurbits up to 1/10,000 dilution [309], and especially IC-PCR has been developed for GLRaV-3 detection in grapevines [310].…”
Section: Immunocapture-pcr (Ic-pcr)mentioning
confidence: 99%