Development of an HPLC Method for the Determination of Glycerol Oxidation Products

Abstract

& An HPLC method for the separation of glycerol oxidation products, namely glyceraldehyde, dihydroxyacetone, mesoxalic, tartronic, and glycolic and glyceric acids on an ion-exchange 8% cross-linked calcium sulfonated divinylbenzene-styrene resin column was developed and validated. The conditions reported include temperature (70 C), flow rate (0.5 mL=min) and concentration of the mobile phase (3 mM H 2 SO 4 ) using isocratic elution with ultraviolet and refractometric detectors. The effect of the mobile phase flow rate and concentration as well as column temperature on the resolution of peaks is described. Excellent correlation coefficient in the calibration model was observed for all analytes over the concentration range of 0.5 to 10 mg=mL. The method was also validated in terms of intra-day precision, sensitivity, accuracy, and detection and quantification limits. The method conditions were applied to the identification of products derived from the chemical oxidation of glycerol.

Superior Electrocatalytic Performance of Au‐Pt Graded Nano‐Alloys Towards Alcohol Oxidation Reaction

“…The chemical name of glycerol is 1, 2, 3 – trihydroxy propane and is available in plenty as a by‐product in soap industries . The electrochemical oxidation of glycerol is not well established.…”

“…The chemical name of glycerol is 1, 2, 3 -trihydroxy propane and is available in plenty as a by-product in soap industries. [42] The electrochemical oxidation of glycerol is not well established. Schnaidt co-workers have studied the oxidation of glycerol by in-situ IR spectroscopy and cyclic voltammetry.…”

Improvement of an Analytical Method Based on HPLC With Refractive Index Detection for the Analysis of Glycerol Oxidation Products

“…Recovery test with sample fortification obtained in the oxidation of glycerol at 3 concentration levels, in triplicate, with the determination of the standard deviation products is in accordance with the routes proposed in the literature for these types of catalytic system. 21,34 Concerning the characterization results, the optimized and validated methodology is efficient and can be applied to follow the evolution of oxidation reactions.…”

“…11 Later studies improved the method, but the focus was on the oxidation of glycerol rather than the use of different types of catalysts and reaction media, and relevant aspects regarding the validation of the methodologies were not addressed. [12][13][14][15][16][17][18][19][20] Among the methods reported so far, only Beltrán-Pietro and co-workers 21 validated the chromatographic method, using an aminex HPX-87C ion exchange column at a temperature of 70 °C, an RID detector (refractive index) coupled to the UV (ultraviolet) detector and a mobile phase composed of 3 mmol L -1 sulfuric acid (H 2 SO 4 , pH 2.00), with a flow rate of 0.5 mL min -1 , an analysis time of 30 minutes. In this case, it was possible to identify six compounds (mesoxalic acid, tartronic acid, glyceraldehyde, glyceric acid, glycolic acid, and dihydroxyacetone) in the working range of 1000-10000 μg mL -1 .…”

Zinc Ferrite Nanoparticles via Coprecipitation Modified Method: Glycerol as Structure Directing and Stabilizing Agent

“…The HPLC method was based on previously published method. 40 The samples S1-gly and S2-gly were washed with 50 mL mobile phase and centrifuged for 7 min at 7500 rpm and then their supernatant were injected onto HPLC system. Glyceraldehyde (2,3-dihydroxypropanal), glyceric acid (2,3-dihydroxypropanoic acid), dihydroxyacetone (1,3-dihydroxypropan-2-one), tartronic acid (2-hydroxypropanedioic acid) and glycolic acid (2-hydroxyethanoic acid) were obtained from Sigma-Aldrich and used as standards.…”