Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Bilkic, Ines; Sotelo, Diana; Anujarerat, Stephanie; Ortiz, Nickolas  R.; Alonzo, Matthew; Khoury, Raven El; Loyola, Carla C; Joddar, Binata

doi:10.1016/j.heliyon.2022.e12250

Cited by 4 publications

(6 citation statements)

References 48 publications

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“…The extent of heterocellular coupling between neuronal and cardiac cell cultures was quantified and depicted in Figure 4E. Our results depict similar trends to those reported by earlier published studies by others and by our own group, and the extent of heterocellular coupling as well as cellular viability was enhanced in the 3D scaffolds in comparison with the 2D cultures (~5%, data not included) [7][8][9]17,[28][29][30]. The characteristic cardiac and neuronal cell phenotypes and morphologies that were involved in the heterocellular cell coupling were confirmed by confocal microscopic imaging via immunostaining.…”

Section: Resultssupporting

confidence: 89%

“…In order to achieve complex geometries through 3D bioprinting, it is essential for the bioink to maintain its structure and stability after printing and crosslinking. To confirm this behavior, we investigated and compared the increase in the storage moduli for both the cardiac and neuronal bioinks after crosslinking, as reported in a previously published study [30]. However, the extrusion-based cardiac bioink demonstrated a higher storage modulus, indicating more elastic-like behavior (G ) [30] when compared with the neuronal bioink [30].…”

Section: Discussionmentioning

confidence: 57%

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A Semi-Three-Dimensional Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System Model

Hernandez,

Ramirez,

Salazar

et al. 2023

Bioengineering

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In this study, we designed a tissue-engineered neurocardiac model to help us examine the role of neuronal regulation and confirm the importance of neural innervation techniques for the regeneration of cardiac tissue. A three-dimensional (3D) bioprinted neurocardiac scaffold composed of a mixture of gelatin–alginate and alginate–genipin–fibrin hydrogels was developed with a 2:1 ratio of AC16 cardiomyocytes (CMs) and retinoic acid-differentiated SH-SY5Y neuronal cells (NCs) respectively. A unique semi-3D bioprinting approach was adopted, where the CMs were mixed in the cardiac bioink and printed using an anisotropic accordion design to mimic the physiological tissue architecture in vivo. The voids in this 3D structure were methodically filled in using a NC–gel mixture and crosslinked. Confocal fluorescent imaging using microtubule-associated protein 2 (MAP-2) and anticholine acetyltransferase (CHAT) antibodies for labeling the NCs and the MyoD1 antibody for the CMs revealed functional coupling between the two cell types in the final crosslinked structure. These data confirmed the development of a relevant neurocardiac model that could be used to study neurocardiac modulation under physiological and pathological conditions.

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Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 57%

A Semi-Three-Dimensional Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System Model

Hernandez,

Ramirez,

Salazar

et al. 2023

Bioengineering

Self Cite

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show abstract

“…In order to achieve complex geometries through 3D bioprinting, it is essential for the bioink to maintain its structure and stability after printing and crosslinking. To confirm this behavior, we investigated and compared the increase in storage moduli for both cardiac and neuronal bioinks after crosslinking, as reported in a previously published study [25]. However, the extrusion-based cardiac bioink demonstrated a higher storage modulus, indicating more elastic-like behavior (G') [25] when compared to the neuronal bioink [25].…”

Section: Discussionmentioning

confidence: 58%

“…To confirm this behavior, we investigated and compared the increase in storage moduli for both cardiac and neuronal bioinks after crosslinking, as reported in a previously published study [25]. However, the extrusion-based cardiac bioink demonstrated a higher storage modulus, indicating more elastic-like behavior (G') [25] when compared to the neuronal bioink [25]. The observed increase in storage modulus in the cardiac bioink can be attributed to a sustained swelling and degradation pattern of the composite neuro-cardiac scaffold in comparison to the neuronal bioink alone.…”

Section: Discussionmentioning

confidence: 75%

A Semi – Three Dimensional (3D) Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System (CANS) Model

Hernandez,

Ramirez,

Salazar

et al. 2023

Preprint

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In this study, we designed a functional neuro-cardiac model to help us examine the role of neuronal regulation and confirm the importance of neural innervation techniques for cardiac tissue regeneration. A three-dimensional (3D) bioprinted neuro-cardiac scaffold composed of a mixture of gelatin-alginate and alginate-genipin-fibrin hydrogels was developed with a 2:1 ratio of AC16 cardiomyocytes (CMs) and retinoic acid differentiated SH-SY5Y neuronal cells (NCs) respectively. A unique semi-3D bioprinting approach was adopted where the CMs were mixed in the cardiac bioink and printed using an anisotropic accordion design to mimic the physiological tissue architecture in vivo. The voids in this 3D structure were methodically filled in using a NCs-gel mixture and cross-linked. Confocal fluorescent imaging using Microtubule-associated protein 2 (MAP-2) antibodies for labeling NCs, and the MyoD1 antibody for CMs revealed functional coupling between the two cell types in the final cross-linked structure. This data confirmed the development of a physiologically functional neuro-cardiac model that can be used to study neuro-cardiac modulation under physiological and pathological conditions.

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“…Furthermore, proteins [ 37 ] and peptides [ 169 ] are incorporated into bioinks. The most frequently used are collagen [ 170 , 171 , 172 ], gelatin and its derivatives [ 87 , 154 , 173 , 174 , 175 ], fibrinogen in soft or hard tissues [ 176 , 177 , 178 ], egg white [ 179 ], etc. In addition, due to superior mechanical properties, synthetic polymers are potential candidates for bioprinting, such as polycaprolactone [ 180 ], poly(ethylene glycol) [ 144 , 145 ], and polyurethanes [ 54 , 181 , 182 ].…”

Section: Rheology As a Prerequisite For Bioink Formulation And Optimi...mentioning

confidence: 99%

Rheology as a Tool for Fine-Tuning the Properties of Printable Bioinspired Gels

Bercea¹

2023

Molecules

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Over the last decade, efforts have been oriented toward the development of suitable gels for 3D printing, with controlled morphology and shear-thinning behavior in well-defined conditions. As a multidisciplinary approach to the fabrication of complex biomaterials, 3D bioprinting combines cells and biocompatible materials, which are subsequently printed in specific shapes to generate 3D structures for regenerative medicine or tissue engineering. A major interest is devoted to the printing of biomimetic materials with structural fidelity after their fabrication. Among some requirements imposed for bioinks, such as biocompatibility, nontoxicity, and the possibility to be sterilized, the nondamaging processability represents a critical issue for the stability and functioning of the 3D constructs. The major challenges in the field of printable gels are to mimic at different length scales the structures existing in nature and to reproduce the functions of the biological systems. Thus, a careful investigation of the rheological characteristics allows a fine-tuning of the material properties that are manufactured for targeted applications. The fluid-like or solid-like behavior of materials in conditions similar to those encountered in additive manufacturing can be monitored through the viscoelastic parameters determined in different shear conditions. The network strength, shear-thinning, yield point, and thixotropy govern bioprintability. An assessment of these rheological features provides significant insights for the design and characterization of printable gels. This review focuses on the rheological properties of printable bioinspired gels as a survey of cutting-edge research toward developing printed materials for additive manufacturing.

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Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells

Cited by 4 publications

References 48 publications

A Semi-Three-Dimensional Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System Model

A Semi-Three-Dimensional Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System Model

A Semi – Three Dimensional (3D) Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System (CANS) Model

Rheology as a Tool for Fine-Tuning the Properties of Printable Bioinspired Gels

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