Development of an ex vivo cellular model of rheumatoid arthritis: Critical role of cd14‐positive monocyte/macrophages in the development of pannus tissue

Abstract

Objective. To establish an ex vivo cellular model of pannus, the aberrant overgrowth of human synovial tissue (ST).Methods. Inflammatory cells that infiltrated pannus tissue from patients with rheumatoid arthritis (RA) were collected without enzyme digestion, and designated as ST-derived inflammatory cells. Single-cell suspensions of ST-derived inflammatory cells were cultured in medium alone. Levels of cytokines produced in culture supernatants were measured using enzyme-linked immunosorbent assay kits. ST-derived inflammatory cells were transferred into the joints of immunodeficient mice to explore whether these cells could develop pannus. CD14 and CD2 cells were depleted by negative selection.Results Conclusion. These findings suggest that overgrowth of inflammatory cells from human rheumatoid synovium simulates the development of pannus. This may prove informative in the screening of potential antirheumatic drugs.

Effects of sesamin on primary human synovial fibroblasts and SW982 cell line induced by tumor necrosis factor-alpha as a synovitis-like model

“…Although animal models can yield an overall understanding of RA pathogenesis, these models also have serious limitations. Due to discrepancies between human arthritis and animal models of arthritis, some responses are different [20]. Importantly, although many drugs have shown great potency in animal models, this advantage has not been borne out in clinical trials [20, 36].…”

“…Due to discrepancies between human arthritis and animal models of arthritis, some responses are different [20]. Importantly, although many drugs have shown great potency in animal models, this advantage has not been borne out in clinical trials [20, 36]. Relatedly, the key point of RA progression is associated with the inflammation of the synovial membrane around the RA joint [3].…”

“…Relatedly, the key point of RA progression is associated with the inflammation of the synovial membrane around the RA joint [3]. Thus, in vitro models that use human cells were developed to study the mechanism of RA and possible therapeutic approaches [20]. Therefore, primary human fibroblast-like synoviocytes from RA patients have been used to screen and study the unique effects of many drugs and phytochemicals [21–23].…”

“…The use of human cells was established to study the mechanism of RA and possible therapeutic approaches [20]. Thus, primary human fibroblast-like synoviocytes isolated from RA patients have been used to study the effects of a variety of drugs and phytochemicals [21–23].…”

Effects of CD14 Macrophages and Proinflammatory Cytokines on Chondrogenesis in Osteoarthritic Synovium-Derived Stem Cells

“…12 It has been previously shown that targeted depletion of synovial macrophages by using anti-CD14-conjugated magnetic beads in RA synovial cells improves inflammation. 13,14 For OA, it has also been shown that OA synovial macrophages likely play an important role in activating the fibroblasts and perpetuating the production of proinflammatory cytokines and destructive enzymes. Although the levels of proinflammatory cytokines are generally lower in OA than those are in RA, IL-1b and TNF-a have been suggested as the key players in OA synovial inflammation.…”