“…The published methods for determination of amphenicols in feeds used the techniques of enzyme-linked immunosorbent assay (ELISA) (16,17), planar chromatography (30), liquid chromatography (13,14), and liquid chromatography tandem mass spectrometry (LC-MS-MS) (3,25). The microbiological technique (9), ELISA (16,17), and a procedure utilising biosensors (15) had to be chemically confirmed. Single quadrupole gas chromatography-mass spectrometry (GC-MS) was also adopted for the determination, confirmation, and concentration measurement of CAP, TAP, FF, and florfenicol amine (FFA) (11,24).…”