Development of an enzyme-linked immunosorbent assay for podophyllotoxin

Approaches for the determination of florfenicol and thiamphenicol in pork using a chemiluminescent ELISA

“…The haptens were synthesized by coupling FF with succinic anhydride (SH), 29 or maleic anhydride (MH). 30 Briey, a solution of FF (3.57 g, 10 mmol) in dry pyridine (30 mL) was added to SH (3.0 g, 30 mmol) at room temperature.…”

Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed1

“…The haptens were synthesized by coupling FF with SH (Diener et al, 1981;Xu et al, 2005) or FF maleate (Krasnova et al, 2001). Briefly, a solution of FF (3.57 g, 10 mmol) in dry pyridine (20 mL) was added to SH (3.0 g, 30 mmol) at room temperature.…”

“…The residue was dissolved with ethyl acetate (30 mL) and then was washed 3 times with hydrochloric acid (0.1 M, 40 mL) followed by purified water (30 mL) for 2 times. The solvent was The haptens were coupled to BSA or HSA by the active eater method (Xu et al, 2005;Luo et al, 2009a) to prepare 4 immunogens (FF-SH-BSA, FF-SH-HSA, FF-MH-BSA, and FF-MH-HSA). Briefly, the hapten (50 mg, 0.11 mmol) dissolved in A^,A'^dimethylformamide (4 mL) was mixed with A''-hydroxysuccinamide (27 mg, 0.23 mmol) and Af,A''-dicyclohexylcarbodiimide (49 mg, 0.24 mmol).…”

Development of an enzyme-linked immunosorbent assay for the detection of florfenicol in fish feed

“…The hapten was coupled to HSA by active eater method to prepare immunogen FF-HSA (Xu et al, 2005). The hapten (50 mg, 0.11 mmol) dissolved in N,Ndimethylformamide (DMF, 4 mL) was mixed with NHS (27 mg, 0.23 mmol) and DCC (49 mg, 0.24 mmol).…”

“…The coating antigen FF-OVA was synthesised by mixed anhydride reaction (Xu et al, 2005). The hapten (50 mg, 0.11 mmol) and tri-n-butylamine (30 mL) were dissolved in DMF (1 mL) and cooled to 08C for 10 min.…”