Development of an Enzyme-Linked Immunosorbent Assay to Detect and Quantify Adenovirus in Chicken Tissues

Saifuddin, Md.; Wilks, C. R.

doi:10.2307/1591404

Cited by 21 publications

(5 citation statements)

References 13 publications

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“…At the end of the incubation period, the cells were frozen and thawed three times, then harvested, along with the culture fluid, for further passage. Each sample was subjected to three blind passages, after which the culture fluid was tested by counterimmunoelectrophoresis (CIE) (14), dot enzyme-linked immunosorbent assay (ELISA) and double antibody sandwich ELISA (16) for the presence of avian adenovirus (AAV) group antigens. Serotyping was performed on ten isolates, using the microneutralisation test in a 96-well tissue culture plate as described by Grimes et al (8).…”

Section: Methodsmentioning

confidence: 99%

Epidemiology of inclusion body hepatitis in poultry in northern India from 1990 to 1994

Singh

Oberoi²,

Jand³

et al. 1996

Rev. Sci. Tech. OIE

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The epidemiology of inclusion body hepatitis (IBH) was studied in poultry in northern India, from April 1990 to March 1994, to evaluate the various factors responsible for causing and determining the severity of the disease. Broiler chicks and Japanese quail (Coturnix coturnix japonica) were the species examined. The factor observed to be most commonly associated with IBH was the presence of aflatoxins in the feed at higher than permissible levels, i.e. 20 parts per billion. Avian adenovirus-1 was isolated from the livers of affected birds. In the final year of the study, a number of outbreaks of IBH caused heavy mortalities among three to five-week-old broiler chicks, which displayed typical IBH lesions in addition to hydropericardium.

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Section: Methodsmentioning

confidence: 99%

Epidemiology of inclusion body hepatitis in poultry in northern India from 1990 to 1994

Singh

Oberoi²,

Jand³

et al. 1996

Rev. Sci. Tech. OIE

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“…Since then, multiple vaccine strains have been selected and tested in trials conducted in South Korea to find suitable candidates for immunoprophylactic purposes. Efforts have also been made to develop vaccines that could confer broad cross-protection against multiple serotypes of FAdVs, as conceptualized based on previous studies [72][73][74]. In 2014, a team developed an inactivated oil emulsion vaccine for FAdV-4 and evaluated it to determine whether any cross-protective immunity was achieved in vaccinated chickens challenged with multiple FAdV serotypes, including their progenies [57].…”

Section: Development Of Domestic Fadv Vaccinesmentioning

confidence: 99%

Historical Investigation of Fowl Adenovirus Outbreaks in South Korea from 2007 to 2021: A Comprehensive Review

2021

Viruses

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Fowl adenoviruses (FAdVs) have long been recognized as critical viral pathogens within the poultry industry, associated with severe economic implications worldwide. This specific group of viruses is responsible for a broad spectrum of diseases in birds, and an increasing occurrence of outbreaks was observed in the last ten years. Since their first discovery forty years ago in South Korea, twelve antigenically distinct serotypes of fowl adenoviruses have been described. This comprehensive review covers the history of fowl adenovirus outbreaks in South Korea and updates the current epidemiological landscape of serotype diversity and replacement as well as challenges in developing effective broadly protective vaccines. In addition, transitions in the prevalence of dominant fowl adenovirus serotypes from 2007 to 2021, alongside the history of intervention strategies, are brought into focus. Finally, future aspects are also discussed.

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“…Saifuddin & Wilks (1990a) have reported an antigen detection ELISA using plates coated with rabbit immunoglobulins. The assay was sen-sitive for antigen detection in infected chicken tissues and cell cultures.…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of monoclonal antibodies to fowl adenoviruses

Ahmad¹,

Burgess

2001

Avian Pathology

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A panel of 10 monoclonal antibodies to a hypervirulent fowl adenovirus (FAdV) strain 398A was produced. Monoclonal antibodies (mAbs) were characterized for their isotype, neutralizing activity, ability to capture viral antigens, immunoblotting of viral polypeptides, competitive inhibition with chicken antisera and their reaction pattern with other FAdVs in indirect immunoperoxidase. Eight out of 10 mAbs reacted strongly in indirect immunoperoxidase staining with most of the FAdVs isolated from inclusion body hepatitis in Australia. One of these mAbs (6E1) was found to specifically react with the strains presumably characterized as hypervirulent FAdVs. IgG 2a was the predominant sub-isotype. Two out of 10 mAbs neutralized the homologous strain of virus and six captured their target antigen onto a plastic surface. Chicken anti-serum to FAdV strain 398A inhibited the reaction of the seven mAbs that bound with high affinity in a blocking competitive enzyme-linked immunosorbent assay. This panel of mAbs can be used to improve diagnostic assays, study pathogenesis and carry out strain identification.

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Development of an Enzyme-Linked Immunosorbent Assay to Detect and Quantify Adenovirus in Chicken Tissues

Cited by 21 publications

References 13 publications

Epidemiology of inclusion body hepatitis in poultry in northern India from 1990 to 1994

Epidemiology of inclusion body hepatitis in poultry in northern India from 1990 to 1994

Historical Investigation of Fowl Adenovirus Outbreaks in South Korea from 2007 to 2021: A Comprehensive Review

Production and characterization of monoclonal antibodies to fowl adenoviruses

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