Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label

Porcheron, P.; Morinière, Madeleine; Grassi, Jacques; Pradelles, Philippe

doi:10.1016/0020-1790(89)90081-4

Cited by 135 publications

(77 citation statements)

References 14 publications

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“…To examine whether NOS activity in the PG reduces ecdysone production, we measured ecdysteroid levels using a competitive enzyme immunoassay (Porcheron et al 1989). In larvae overexpressing NOS in the PG (phm .…”

Section: Growth Of Imaginal Tissues Is Selectively Regulated During Tmentioning

confidence: 99%

Nitric Oxide Synthase Regulates Growth Coordination DuringDrosophila melanogasterImaginal Disc Regeneration

et al. 2015

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Mechanisms that coordinate growth during development are essential for producing animals with proper organ proportion. Here we describe a pathway through which tissues communicate to coordinate growth. During Drosophila melanogaster larval development, damage to imaginal discs activates a regeneration checkpoint through expression of Dilp8. This both produces a delay in developmental timing and slows the growth of undamaged tissues, coordinating regeneration of the damaged tissue with developmental progression and overall growth. Here we demonstrate that Dilp8-dependent growth coordination between regenerating and undamaged tissues, but not developmental delay, requires the activity of nitric oxide synthase (NOS) in the prothoracic gland. NOS limits the growth of undamaged tissues by reducing ecdysone biosynthesis, a requirement for imaginal disc growth during both the regenerative checkpoint and normal development. Therefore, NOS activity in the prothoracic gland coordinates tissue growth through regulation of endocrine signals.KEYWORDS regeneration; nitric oxide; ecdysone; allometry; growth control A LLOMETRY, broadly defined as the scaling of organ growth, can have a profound impact on the biological function in animals. For example, in the male dung beetle, Onthophagus netriventris, an inverse allometry is observed between horn and testes size, producing distinct reproductive strategies (Simmons and Emlen 2006;Emlen et al. 2012). Allometric growth regulation can also impact human health where variation from optimal relative heart size can increase susceptibility to cardiovascular disease (Hill and Olson 2008). Despite the fundamental role of growth scaling in biology, no described pathways explain how tissues coordinate growth. Our understanding of growth regulation has been focused on either tissue-autonomous mechanismssuch as how morphogens regulate the activity of cellular growth pathways-or systemic mechanisms such as how endocrine factors control growth in response to environmental change.These tissue-autonomous and systemic pathways of growth regulation do not explain allometric growth observed during development. Transplantation experiments (Madhavan and Schneiderman 1969) and growth perturbation experiments in Drosophila and other insects (Nijhout and Emlen 1998;Simmons and Emlen 2006;Parker and Shingleton 2011) suggest that interorgan communication may be necessary for allometric growth. Based on these observations, Stern and Emlen (1999) proposed a model for growth coordination that requires communication between growing organs, either directly or indirectly through an endocrine organ. However, the mechanism of this communication pathway has been unclear.In Drosophila larvae, the growth of the imaginal discs is tightly regulated to produce adult structures with specific size and proportion (Mirth and Shingleton 2012;Callier and Nijhout 2013). Allometry between these tissues is preserved even when developmental growth programs are altered. For example, Drosophila imaginal discs have a remar...

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Section: Growth Of Imaginal Tissues Is Selectively Regulated During Tmentioning

confidence: 99%

Nitric Oxide Synthase Regulates Growth Coordination DuringDrosophila melanogasterImaginal Disc Regeneration

et al. 2015

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“…Hemolymph ecdysteroids were quantified by enzyme-linked immunosorbent assay following the procedure described by Porcheron et al (1989), and adapted to B. germanica by Pascual et al (1994) and Romañ á et al (1995). The ecdysteroid antiserum has the same affinity for ecdysone and 20E (Porcheron et al, 1989), but because the standard curve was obtained with the latter compound, results are expressed as 20E equivalents.…”

Section: Quantification Of Ecdysteroidsmentioning

confidence: 99%

“…The ecdysteroid antiserum has the same affinity for ecdysone and 20E (Porcheron et al, 1989), but because the standard curve was obtained with the latter compound, results are expressed as 20E equivalents.…”

Section: Quantification Of Ecdysteroidsmentioning

confidence: 99%

Nuclear receptor BgFTZ‐F1 regulates molting and the timing of ecdysteroid production during nymphal development in the hemimetabolous insect Blattella germanica

Cruz¹,

Nieva²,

Mané-Padrós³

et al. 2008

Developmental Dynamics

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“…The ecdysteroid level in the haemolymph was measured using enzyme-immunoassay (EIA) as described by Porcheron et al (1989). Haemolymph was collected from larvae via an incision in their proleg then mixed with methanol (1 : 9) before centrifugation at 10,000 × g for 10 min.…”

Section: Measurement Of the Level Of Ecdysteroid In Haemolymph Using mentioning

confidence: 99%

A comprehensive study of the changes in ecdysteroid levels during the feeding phase of fifth instar larvae of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Keshan¹,

Thounaojam²,

Kh.³

2015

Eur. J. Entomol.

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Abstract. The present study describes changes in haemolymph ecdysteroid levels during the feeding phase of fifth instar larvae of Bombyx mori. A small and sharp peak in ecdysteroid level was recorded on day 4 when larvae had already reached the critical weight (2.76 ± 0.11 g) for metamorphosis. Food deprivation of larvae from day 4 resulted in an increase in the ecdysteroid level. Injection of insulin in both 1 day (before reaching critical weight) and 4 day (after reaching critical weight) old fed larvae resulted in an increase in the ecdysteroid level. However, when deprived of food only the haemolymph ecdysteroid levels of 4 day old larvae respond to an injection of insulin. The hormone level was also increased by treating 1day old larvae with a juvenile hormone analogue, but again, like insulin, only the fed larvae were affected by this treatment. Neck-and thorax-ligated 4 day old larvae had decreased ecdysteroid levels compared to both fed and food deprived larvae. However, a significant increase in ecdysteroid level was recorded in both neck-and thorax-ligated larvae injected with insulin. The injection of 20-hydroxyecdysone into 4 day old larvae resulted in an increase in the level of this substance in the haemolymph of fed and food deprived larvae, but the level was significantly higher in neck-and thoraxligated la rvae. The data, thus, indicates that ecdysteroid levels vary both in relation to the critical weight of the larvae and its nutritional status, and insulin might also play an important role in regulating the level of ecdysteroid.

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Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label

Cited by 135 publications

References 14 publications

Nitric Oxide Synthase Regulates Growth Coordination DuringDrosophila melanogasterImaginal Disc Regeneration

Nitric Oxide Synthase Regulates Growth Coordination DuringDrosophila melanogasterImaginal Disc Regeneration

Nuclear receptor BgFTZ‐F1 regulates molting and the timing of ecdysteroid production during nymphal development in the hemimetabolous insect Blattella germanica

A comprehensive study of the changes in ecdysteroid levels during the feeding phase of fifth instar larvae of the silkworm, Bombyx mori (Lepidoptera: Bombycidae)

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