Development of an electrochemical genosensor for detection of viral hemorrhagic septicemia virus (VHSV) using glycoprotein (G) gene probe

Moattari, Ghazal; Izadi, Zahra; Shakhsi-Niaei, Mostafa

doi:10.1016/j.aquaculture.2021.736451

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Electrochemical genetic sensors allow for rapid, inexpensive, and sensitive detection of fish diseases. In most studies, fish diseases are detected primarily through electrochemical DNA sensors [ 140 ]. In contrast, water-quality sensors can detect changes in the water environment by detecting dissolved oxygen, pH, water temperature, and other changes in the water that may be affected by fish health aspects, but not specific diseases.…”

Section: Intelligent Diagnosis Methods Of Fish Diseases Based On Imagesmentioning

confidence: 99%

Advanced Techniques for the Intelligent Diagnosis of Fish Diseases: A Review

Wang

et al. 2022

Animals

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Aquatic products, as essential sources of protein, have attracted considerable concern by producers and consumers. Precise fish disease prevention and treatment may provide not only healthy fish protein but also ecological and economic benefits. However, unlike intelligent two-dimensional diagnoses of plants and crops, one of the most serious challenges confronted in intelligent aquaculture diagnosis is its three-dimensional space. Expert systems have been applied to diagnose fish diseases in recent decades, allowing for restricted diagnosis of certain aquaculture. However, this method needs aquaculture professionals and specialists. In addition, diagnosis speed and efficiency are limited. Therefore, developing a new quick, automatic, and real-time diagnosis approach is very critical. The integration of image-processing and computer vision technology intelligently allows the diagnosis of fish diseases. This study comprehensively reviews image-processing technology and image-based fish disease detection methods, and analyzes the benefits and drawbacks of each diagnostic approach in different environments. Although it is widely acknowledged that there are many approaches for disease diagnosis and pathogen identification, some improvements in detection accuracy and speed are still needed. Constructing AR 3D images of fish diseases, standard and shared datasets, deep learning, and data fusion techniques will be helpful in improving the accuracy and speed of fish disease diagnosis.

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Section: Intelligent Diagnosis Methods Of Fish Diseases Based On Imagesmentioning

confidence: 99%

Advanced Techniques for the Intelligent Diagnosis of Fish Diseases: A Review

Wang

et al. 2022

Animals

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“…The first example of an electrochemical genosensor has been reported for the detection of the VHSV Glycoprotein gene, using a PGE modified with a nanocomposite including reduced graphene oxide (rGO) and AuNPs (Au/rGO) [99]. The DNA probe was immobilized onto the modified electrode through thiol chemistry.…”

Section: 28 3777mentioning

confidence: 99%

Functional Nanomaterials Enhancing Electrochemical Biosensors as Smart Tools for Detecting Infectious Viral Diseases

Curulli

2023

Molecules

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Electrochemical biosensors are known as analytical tools, guaranteeing rapid and on-site results in medical diagnostics, food safety, environmental protection, and life sciences research. Current research focuses on developing sensors for specific targets and addresses challenges to be solved before their commercialization. These challenges typically include the lowering of the limit of detection, the widening of the linear concentration range, the analysis of real samples in a real environment and the comparison with a standard validation method. Nowadays, functional nanomaterials are designed and applied in electrochemical biosensing to support all these challenges. This review will address the integration of functional nanomaterials in the development of electrochemical biosensors for the rapid diagnosis of viral infections, such as COVID-19, middle east respiratory syndrome (MERS), influenza, hepatitis, human immunodeficiency virus (HIV), and dengue, among others. The role and relevance of the nanomaterial, the type of biosensor, and the electrochemical technique adopted will be discussed. Finally, the critical issues in applying laboratory research to the analysis of real samples, future perspectives, and commercialization aspects of electrochemical biosensors for virus detection will be analyzed.

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“…The detection of viral hemorrhagic septicemia (VHS), a very common infection in water and fish, has been investigated [61] . According to the authors, RT-PCR and real-time PCR are the two most common detection tools for VHS due to their rapidness and sensitivity.…”

Section: Biosensors For the Detection Of Pathogensmentioning

confidence: 99%

“…However, they are not cost-effective and require professional operation inside well-equipped laboratories. An electrochemical biosensor was developed by Moattari et al through the immobilization of VHS-specific DNA probes on pencil graphite electrodes [61] . Using methylene blue, several DNA sequences were investigated on the reported sensor and high-sensitivity results were obtained.…”

Section: Biosensors For the Detection Of Pathogensmentioning

confidence: 99%