Development of an electro-responsive platform for the controlled transfection of mammalian cells

Hook, Andrew L.; Thissen, Helmut; Hayes, Jason P.; Voelcker, Nicolas H.

doi:10.1117/12.606789

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…The preferential adsorption of the DNA to the ALAPP regions (green regions) as opposed to the PEO regions (dark regions) without the application of a voltage was observed (Fig. 5A), demonstrating the spatially controlled adsorption of DNA (Hook et al, 2005). We propose that the mechanism for this adsorption is due mainly to electrostatic interactions of surface-tethered, protonated amino groups with the negatively charged backbone of DNA.…”

Section: Dna Adsorption and Desorption Studiesmentioning

confidence: 79%

Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

Hook

Thissen

Hayes

et al. 2006

Biosensors and Bioelectronics

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Section: Dna Adsorption and Desorption Studiesmentioning

confidence: 79%

Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

Hook

Thissen

Hayes

et al. 2006

Biosensors and Bioelectronics

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“…A platform for cell arrays has recently been developed by the creation of a two-dimensional chemical pattern, formed by the sequential deposition of an allylamine plasma polymer (ALAPP), poly(ethylene glycol) (PEG) grafting and laser ablation [20][21][22][23][24] . Using this platform spatial control over the adsorption of cells 21 and DNA 20 has been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

A platform for the advanced spatial and temporal control of biomolecules

et al. 2006

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Manipulating biomolecules at solid/liquid interfaces is important for the development of various biodevices including microarrays. Smart materials that enable both spatial and temporal control of biomolecules by combining switchability with patterned surface chemistry offer unprecedented levels of control of biomolecule manipulation. Such a system has been developed for the microscale spatial control over both DNA and cell growth on highly doped p-type silicon. Surface modification, involving plasma polymerisation of allylamine and poly(ethlylene glycol) grafting with subsequent laser ablation, led to the production of a patterned surface with dual biomolecule adsorption and desorption properties. On patterned surfaces, preferential electro-stimulated adsorption of DNA to the allylamine plasma polymer surface and subsequent desorption by the application of a negative bias was observed. The ability of this surface to control both DNA and cell attachment in four dimensions has been demonstrated, exemplifying its capacity to be used for complex biological studies such as gene function analysis. This system has been successfully applied to living microarray applications and is an exciting platform for any system incorporating biomolecules.

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Development of an electro-responsive platform for the controlled transfection of mammalian cells

Cited by 2 publications

References 21 publications

Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

A platform for the advanced spatial and temporal control of biomolecules

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