Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

Hook, Andrew L.; Thissen, Helmut; Hayes, Jason P.; Voelcker, Nicolas H.

doi:10.1016/j.bios.2005.10.008

Cited by 39 publications

(49 citation statements)

References 29 publications

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“…In order to produce a PEG modified surface, a methodology has been proposed by the groups of Griesser, Thissen and Voelcker in which a PEG layer is reacted with an amine plasma polymer coated slide. [51][52][53][54]. Of importance was the production of a dense, brush-like layer, produced by grafting the PEG to the surface at 'cloud point' conditions in order to optimise the ability of this layer to resist the adsorption of biomolecules.…”

Section: Slide Coatingsmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

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The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the literature in this area and conclude that for polymers, this process is best achieved in a microarray format, which enable thousands of cell-material interactions to be monitored on a single chip. Polymer microarrays can be formed by printing pre-synthesised polymers or by printing monomers onto the chip where on-slide polymerisation is initiated.The surface properties of the material can be analysed and correlated to the biological performance using high throughput surface analysis, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), Xray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. This approach enables the surface properties responsible for the success of a material to be understood, which in turn provides the foundations of future material design. The high throughput discovery of materials using polymer microarrays has been explored for many cell-based applications including the isolation of specific 2 cells from heterogeneous populations, the attachment and differentiation of stem cells and the controlled transfection of cells.Further development of polymerisation techniques and high throughput biological assays amenable to the polymer microarray format will broaden the combinatorial space and biological phenomenon that polymer microarrays can explore, and increase their efficacy. This will, in turn, result in the discovery of optimised polymeric materials for many biomaterial applications.

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Section: Slide Coatingsmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

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“…DNA adsorption studies were conducted in a custom-built flow cell previously described 20 in conjunction with a Leitz Fluorescence microscope. Patterned ALAPP modified PEG grafted samples were clamped into the flow cell and 200 µl of ultra pure water was injected over the sample.…”

Section: Dna Adsorption Studiesmentioning

confidence: 99%

“…A platform for cell arrays has recently been developed by the creation of a two-dimensional chemical pattern, formed by the sequential deposition of an allylamine plasma polymer (ALAPP), poly(ethylene glycol) (PEG) grafting and laser ablation [20][21][22][23][24] . Using this platform spatial control over the adsorption of cells 21 and DNA 20 has been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

“…Using this platform spatial control over the adsorption of cells 21 and DNA 20 has been demonstrated. The use of plasma polymerisation enables the modification of almost all substrate materials with an adherent polymer layer that has a specific chemical functionality depending on the choice of monomer.…”

Section: Introductionmentioning

confidence: 99%

“…This is also a solvent free process, which prevents the presence of potentially toxic residual solvent molecules, increasing the biocompatibility of this material. Allylamine was chosen in the present case because of its amine functionality, which assists with cell attachment 25 , DNA adsorption 20 and enables the covalent immobilisation of PEG, which remains unrivalled in its ability to prevent bio-fouling 5 . Laser ablation is finally used to remove regions of the PEG and re-expose the underlying ALAPP layer to create biologically active wells within a non-fouling surface.…”

Section: Introductionmentioning

confidence: 99%

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A platform for the advanced spatial and temporal control of biomolecules

et al. 2006

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Manipulating biomolecules at solid/liquid interfaces is important for the development of various biodevices including microarrays. Smart materials that enable both spatial and temporal control of biomolecules by combining switchability with patterned surface chemistry offer unprecedented levels of control of biomolecule manipulation. Such a system has been developed for the microscale spatial control over both DNA and cell growth on highly doped p-type silicon. Surface modification, involving plasma polymerisation of allylamine and poly(ethlylene glycol) grafting with subsequent laser ablation, led to the production of a patterned surface with dual biomolecule adsorption and desorption properties. On patterned surfaces, preferential electro-stimulated adsorption of DNA to the allylamine plasma polymer surface and subsequent desorption by the application of a negative bias was observed. The ability of this surface to control both DNA and cell attachment in four dimensions has been demonstrated, exemplifying its capacity to be used for complex biological studies such as gene function analysis. This system has been successfully applied to living microarray applications and is an exciting platform for any system incorporating biomolecules.

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Ultrathin Functional Polymer Films Using Plasma‐Assisted Deposition

Förch

2011

Functional Polymer Films

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Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications

Cited by 39 publications

References 29 publications

High throughput methods applied in biomaterial development and discovery

High throughput methods applied in biomaterial development and discovery

A platform for the advanced spatial and temporal control of biomolecules

Ultrathin Functional Polymer Films Using Plasma‐Assisted Deposition

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