Development of an Efficient Method to Extract DNA from Refined Soybean Oil

Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs)

“…The results of this research show that DNA fragments can be identified and measured in some oil samples, and this result is in agreement with the research of some researchers ( Hellebrand et al, 1998 ; Zhang et al, 2007; Schmidt et al, 2008 , Costa et al, 2010 , Costa et al, 2010 , Luna et al, 2013 , Nikolić et al, 2014 , Shayan et al, 2017 , Iioh et al, 2018 , Sepahian et al, 2019 , Piarulli et al, 2019 , Fröder et al, 2020 , Xia et al, 2021 ). Also, the investigation of some researchers indicates that due to the destruction of DNA in the oil refining process, it is not possible to identify GMO products in the oil, and there is no need for labeling ( Pauli et al, 1998 , Gryson et al, 2002 , Gryson et al, 2004a , Gryson et al, 2004b , Costa et al, 2010 , Costa et al, 2010 , Jinxia et al, 2011 ).…”

“…In general, detection methods for GM products can be divided into two main categories: DNA-based methods and protein-based methods ( Xia et al, 2021 ). The application of a DNA-based method for the detection and quantification of GMOs and achieving successful results in DNA amplification methods depends on the efficiency of DNA extraction methods and, consequently, the quality and quantity of the extracted DNA.…”

Soybean Oil: A Versatile Asset in Pharmaceutical Drug Delivery

“…After compactification of crushing or flaking, beans undergo a conditioning process. Oils were separated from the cellular structure when beans were at the appropriate temperature [25]. In this step, extraction efficiency was boosted, and quality was also achieved.…”

A review on the identification of transgenic oilseeds and oils

“…PCR is the most widely used method for nucleic acid detection because of its higher specificity and accuracy (Qian et al., 2018). It was a proven fact that the extraction of DNA was vital to the success of PCR and its related technologies, especially for highly processed oils (Xia et al., 2020). In order to satisfy the determination requirements of consumers and industry regulators, nucleic acid fragments have been successfully extracted from the refined transgenic oils (Duan et al., 2021), and several qualitative/quantitative PCR and related technologies have been applied to detect nucleic acid elements in oils (Xia et al., 2021).…”