Development of an amperometric enzyme electrode biosensor for sterigmatocystin detection

Chen, Junhua; Liu, Daling; Li, Shichuan; Yao, Dongsheng

doi:10.1016/j.enzmictec.2010.06.008

Cited by 38 publications

(21 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An enzyme extract from the edible mushroom Armillariella tabescens has been reported to degrade AFB1 . The enzyme was subsequently identified and named aflatoxin oxidase (AFO; previous names include aflatoxin‐detoxifyzime and aflatoxin‐oxidoreductase) . The genetic studies showed that aflatoxin‐detoxifizyme gene derived from A. tabescens decreased negative effects of aflatoxins on transgenetic mice .…”

Section: Introductionmentioning

confidence: 99%

“…The genetic studies showed that aflatoxin‐detoxifizyme gene derived from A. tabescens decreased negative effects of aflatoxins on transgenetic mice . The proposed AFB1 degradation mechanism includes unsaturated carbon–carbon bond oxidation to an epoxide intermediate accompanied by H 2 O 2 production . The epoxide is then hydrolyzed to a diol, and the bifuran ring is ultimately opened in a separate hydrolysis step (Figure ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidase or peptidase? A computational insight into a putative aflatoxin oxidase from Armillariella tabescens

Tomin

Tomić

2019

Proteins

View full text Add to dashboard Cite

Aflatoxin oxidase (AFO), an enzyme isolated from Armillariella tabescens, has been reported to degrade aflatoxin B1 (AFB1). However, recent studies reported sequence and structure similarities with the dipeptidyl peptidase III (DPP III) family of enzymes and confirmed peptidase activity toward DPP III substrates. In light of these investigations, an extensive computational study was performed in order to improve understanding of the AFO functions. Steered MD simulations revealed long‐range domain motions described as protein opening, characteristic for DPPs III and necessary for substrate binding. Newly identified open and partially open forms of the enzyme closely resemble those of the human DPP III orthologue. Docking of a synthetic DPP III substrate Arg2‐2‐naphthylamide revealed a binding mode similar to the one found in crystal structures of human DPP III complexes with peptides with the S1 and S2 subsites’ amino acid residues conserved. On the other hand, no energetically favorable AFB1 binding mode was detected, suggesting that aflatoxins are not good substrates of AFO. High plasticity of the zinc ion coordination sphere within the active site, consistent with that of up to date studied DPPs III, was observed as well. A detailed electrostatic analysis of the active site revealed a predominance of negatively charged regions, unsuitable for the binding of the neutral AFB1. The present study is in line with the most recent experimental study on this enzyme, both suggesting that AFO is a typical member of the DPP III family.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oxidase or peptidase? A computational insight into a putative aflatoxin oxidase from Armillariella tabescens

Tomin

Tomić

2019

Proteins

View full text Add to dashboard Cite

show abstract

“…For ST biosensor, the combination of chitosan and SWCNT provided a microenvironment favourable to keep the native conformation of AFO as well as its functionality, while accelerating the direct electron transfer between the enzyme and the electrode surface. The LOD for ST was comparable to those achieved by GC-MS and LC-MS. 36 The AFO-based sensor for AFB1 determination combined silica sol-gel and MWNTs. 37 MWCNTs acted as supports for the covalent immobilisation of large amounts of enzyme, due to their high specific surface area.…”

Section: Carbon Nanotubes Based Biosensors For Mycotoxins Detectionmentioning

confidence: 99%

“…Aflatoxin-oxidase (AFO) has been used to develop enzyme sensors for sterigmatocystin (ST) 36 and AFB1. 37 In these works, focus was on finding optimum materials for enzyme immobilisation.…”

Section: Carbon Nanotubes Based Biosensors For Mycotoxins Detectionmentioning

confidence: 99%

Biosensors for Food Toxin Detection: Carbon Nanotubes and Graphene

2015

View full text Add to dashboard Cite

There is increased interest towards the application of carbon based nanomaterials to biosensors since these can be used to quickly detect presence of the toxins in food, agricultural and environmental systems. The accurate, faster and early detection of food toxins is presently very important for ensuring safety and shelf life of agricultural commodities resulting from food contamination. The carbon materials (CNTs) and recently discovered graphene have been predicted to be promising candidates in the development of electrochemical biosensor owing to their exceptionally large surface area and interesting electrochemical properties. We focus on some of the recent results obtained in our laboratories pertaining to the development of biosensors based on multi-walled carbon nanotubes and graphene for mycotoxin(aflatoxin ) detection.

show abstract

“…Aflatoxin-oxidase (AFO), confirmed to possess oxidation activity toward sterigmatocystin, was utilized as bio-recognition element to constructing the enzymatic biosensor for sterigmatocystin detection. Our previously reported AFO biosensors for fast detection of sterigmatocystin have indicated their potential practicability (Yao et al, 2006;Chen et al, 2010). However, to develop anti-interference enzymatic biosensor for the practical food samples is an arduous target.…”

Section: Enzymatic Sensory Detection Of Sterigmatocystinmentioning

confidence: 99%

Enzymatic Sensor for Sterigmatocystin Detection and Feasibility Investigation of Predicting Aflatoxin B1 Contamination by Indicator

Liu¹,

Tan²,

Chen³

et al. 2011

Aflatoxins - Detection, Measurement and Control

View full text Add to dashboard Cite

Development of an amperometric enzyme electrode biosensor for sterigmatocystin detection

Cited by 38 publications

References 33 publications

Oxidase or peptidase? A computational insight into a putative aflatoxin oxidase from Armillariella tabescens

Oxidase or peptidase? A computational insight into a putative aflatoxin oxidase from Armillariella tabescens

Biosensors for Food Toxin Detection: Carbon Nanotubes and Graphene

Enzymatic Sensor for Sterigmatocystin Detection and Feasibility Investigation of Predicting Aflatoxin B1 Contamination by Indicator

Contact Info

Product

Resources

About