Development of an algorithm of satellite markers for monitoring chimerism status in post-allogeneic haematopoietic stem cell transplantation patients

Abstract

Since population genetic studies on VNTR loci are not well established in Southeast Asia, the present study is useful to determine reliable markers during the initial screening step of chimerism analysis. By following this algorithm, we are able to reduce time and cost of finding a suitable VNTR marker in our cohort.

Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods

“…11,13 For all these clinical applications, the optimal methodological approach needs to be informative, sensitive, quantitatively accurate, reproducible and cost effective. [13][14][15][16] In the present study, we compared and validated chimerism in pediatric recipients of post-HSCT between two methods: quantitative real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR) to find a more accurate and efficient methodology to asses chimerism.…”

Chimerism analyses by sex-chromosome analysis confused relapse with donor cell leukemia after sex-mismatched allo-SCT

“…Furthermore, VNTR analysis revealed the recipient pattern and is considered to be the most sensitive technique for discriminating between individuals, as previously reported. 5 These findings suggested leukemic cells of recipient origin. It is therefore significant to consider all these analyses when investigating ALL relapse.…”

Genetic polymorphism study at D1S80 VNTR in four major tribal populations (Garo, Santal, Khasia and Monipuri) of Bangladesh

“…Genetic data is also scarce to portray the genetic structure of these populations. D1S80 is variable number of tandem repeat (VNTR) locus positioned in the telomeric region of chromosome 1 at 1p36-p35 with a core repeat unit of 16-nucleotides (Choong et al, 2011;Fujii et al, 2004;Herrera et al, 2004;Mukherjee et al, 2005;Verbenko et al, 2006). Like an ideal VNTR marker the D1S80 locus shows a high degree of polymorphism as well as heterozygosity (Herrera et al, 2004).…”

“…Like an ideal VNTR marker the D1S80 locus shows a high degree of polymorphism as well as heterozygosity (Herrera et al, 2004). This makes D1S80 a highly useful genetic marker in population diversity studies (Choong et al, 2011;Fujii et al, 2004;Herrera et al, 2004;Mukherjee et al, 2005;Verbenko et al, 2006). D1S80 locus has no known genetic function, and therefore, is supposed to be under no selection pressure (Herrera et al, 2004).…”