Development of a whole liquid egg certified reference material for accurate measurement of enrofloxacin residue

Yang, Mujie; Liu, Fang; Wang, Min; Jian, Zhou; Zhang, Liyuan; Wang, Tongtong

doi:10.1016/j.foodchem.2019.125253

Cited by 21 publications

(4 citation statements)

References 29 publications

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“…This indicates that neither the addition of the tea tree essential oil nor the antibiotics had expressed their negative influence on the fresh table eggs' homogeneity. The use of enrofloxacin residues in eggs and their influence on homogeneity and long-term stability were investigated [80]. The average values obtained in our study recorded a score of around 4, which indicated an "almost homogenous" description of the fresh table eggs, which presented an indicator of good sensory descriptive quality.…”

Section: Resultsmentioning

confidence: 63%

Influence of Different Tetracycline Antimicrobial Therapy of Mycoplasma (Mycoplasma synoviae) in Laying Hens Compared to Tea Tree Essential Oil on Table Egg Quality and Antibiotic Residues

Puvača

Lika

Tufarelli

et al. 2020

Foods

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The food of animal origin that is the most consumed is the table egg, but laying hens treated with antibiotics can produce eggs contaminated with antibiotic residues. Residues of antibiotics may present a risk for consumer health. Keeping in mind that laying hens almost always suffer from Mycoplasma (Mycoplasma synoviae), for which they are treated with antibiotics, high-quality egg production is even harder. Our research aimed to investigate the influence of three different antibiotics compared to the tea tree (Melaleuca alternifolia) essential oil administered to naturally infected laying hens with M. synoviae, on antibiotic residues in eggs as well as the egg nutritive and sensory qualities. A total of 20,000 laying hens, housed in one facility and divided into four lines each consisting of 5000 hens naturally infected with M. synoviae, was used. For the antimicrobial therapy, tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) were used, respectively. As a control, tea tree essential oil (TT) was used. Based on the gained results all tetracyclines treatment residue values were significantly (p < 0.05) higher compared to the control treatment (TT), but without any significant differences (p > 0.05) between themselves. The results showed no differences in the nutritive and the sensory qualities of eggs between the control and the experimental treatments (p > 0.05). Keeping in mind the obtained results from this study, it can be concluded that tea tree essential oil could be successfully used as a natural antibiotic in the treatment of M. synoviae, without any adverse effects on table egg quality.

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Section: Resultsmentioning

confidence: 63%

Influence of Different Tetracycline Antimicrobial Therapy of Mycoplasma (Mycoplasma synoviae) in Laying Hens Compared to Tea Tree Essential Oil on Table Egg Quality and Antibiotic Residues

Puvača

Lika

Tufarelli

et al. 2020

Foods

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“…When the drug dosage increased from SD to LD 50 , the ENR elimination time in brain, kidney, and muscle all delayed, but shortened in the liver, which showed that ENR absorption and metabolism time in crucian carp were related to the drug dosage. At 960 h, the residue of ENR at LD 50 in the brain was up to 0.15 μg/g, which beyond the ENR residue limit of 0.10 μg/g in animal edible tissues approved by China (Yang et al, 2020). At SD, the maximum residues in the brain reached the same time as the liver, but earlier than the kidney and muscle.…”

Section: Discussionmentioning

confidence: 89%

Metabolism and residue differences of Enrofloxacin between the brain and peripheral tissues and the resulting brain damages in crucian carp (Carassius auratus var. Pengze)

Wan

Zhang

Wang

et al. 2022

Vet Pharm & Therapeutics

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This study aimed to explore the metabolism and residue differences of Enrofloxacin (ENR) at two doses between the brain and peripheral tissues (liver, kidney, and muscle) along with the brain damages caused by ENR in crucian carp (Carassius auratus var. Pengze). The concentrations of ENR in tissues were determined using a validated high‐performance liquid chromatography (HPLC) analysis. Relying on the hematoxylin–eosin (HE) staining method, brain damages caused by the drug were evaluated by the section of pathological tissue. Metabolism and residue results showed that ENR could be detected in the brain throughout the experiment both at median lethal dose (LD50 at 96 h, 1949.84 mg/kg) and safe dose (SD, 194.98 mg/kg), as well as in the three peripheral tissues. The maximum residue at LD50 followed the decreasing order of liver >kidney > brain > muscle. Although the Cmax of ENR at SD in the brain was significantly lower than that in other peripheral tissues (p < .05), it still reached 41.91 μg/g. The T1/2 of ENR in brain tissue at the same dose was both shorter than that in peripheral tissues. At LD50, the amount of ENR residues in brain was lower than that in peripheral tissues on the whole, except that it had been higher than in the muscle for the first 3 h. At SD, the drug residue in brain tissue was lower than that in peripheral tissues from 12 h to 960 h, but it exceeded the muscle and kidney at 1 h and 6 h, respectively. At 960 h, the residual amount of ENR at SD in the brain was 0.09 μg/g, while it was up to 0.15 μg/g following the oral administration at LD50. Demonstrated by the HE staining, there were pathological lesions caused by ENR in the brain at LD50, which were characterized by sparse neural network and increased staining of glial cells. The present results indicated that metabolism and residue of ENR in crucian carp were affected by the tissue type and drug dosage, and the ENR could also bring about histopathological changes in the brain.

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“…Matrix CRMs are usually selected to be similar to the test sample in real state, and the value was accurately characterized [25]. Therefore, in recent years, plenty of matrix certified reference materials have been developed, such as ractopamine or salbutamol in mutton powder [26,27], enrofloxacin in whole liquid egg [28], and docosahexaenoic acid in milk powder [29], as well as florfenicol in shrimp [30] for food quality and safety monitoring.…”

Section: Introductionmentioning

confidence: 99%

Certification of a novel matrix reference material for accurate measurements of aflatoxin M1 in milk powder

Huang,

Liu,

Zhou

et al. 2024

Accred Qual Assur

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In this study, two different concentrations of matrix certified reference materials (CRMs) were produced for the accurate measurement of aflatoxin M1(AFM1) in milk powder((GBW(E) 100552, GBW(E) 100553)). The raw material was obtained by feeding cows with positive drugs. The homogeneity, stability and characterization of this matrix CRMs were examined by liquid chromatography tandem mass spectrometry with isotope-labeled internal standard method. The certified value for the low concentration of AFM1 in milk powder was 2.45 µg/kg with an uncertainty of 0.43 µg/kg (coverage factor k=2, at 95% confidence). The certified value for the high concentration of AFM1 in milk powder was 3.45 µg/kg with an expanded uncertainty of 0.49 µg/kg (coverage factor k=2, at 95% confidence). In addition, the samples were evaluated in detail for homogeneity, long-term stability at -80°C for 6 months, and short-term stability at 4°C for 7 days. The results showed that the samples were stable under the above conditions.

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Development of a whole liquid egg certified reference material for accurate measurement of enrofloxacin residue

Cited by 21 publications

References 29 publications

Influence of Different Tetracycline Antimicrobial Therapy of Mycoplasma (Mycoplasma synoviae) in Laying Hens Compared to Tea Tree Essential Oil on Table Egg Quality and Antibiotic Residues

Influence of Different Tetracycline Antimicrobial Therapy of Mycoplasma (Mycoplasma synoviae) in Laying Hens Compared to Tea Tree Essential Oil on Table Egg Quality and Antibiotic Residues

Metabolism and residue differences of Enrofloxacin between the brain and peripheral tissues and the resulting brain damages in crucian carp (Carassius auratus var. Pengze)

Certification of a novel matrix reference material for accurate measurements of aflatoxin M1 in milk powder

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