Development of a Validated Stability- Indicating HPTLC Method for Nitazoxanide in Pharmaceutical Formulation

Ahir, Keyur B.; Patelia, Emanuel M; Mehta, Falgun A.

doi:10.4172/2157-7064.1000138

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…It was discovered in 1984 and classified as a first class of thiazolide. Primarily it was developed as a veterinary helminthic, but also proved effective on many human pathogens [1,2]. Later, NTZ was approved for treating diarrhea caused by the protozoa Cryptosporidium in children and adults.…”

Section: Introductionmentioning

confidence: 99%

“…NTZ is also an antiparasitic drug, which is also effective against a wide variety of helminthes, Gramnegative organisms, and protozoa. Thus, it is effective against a broad range of protozoal, viral, bacterial, and parasitic infections [1,4]. NTZ also possesses high antitumor activity reported against animal tumor models as well as various cancerous cell lines.…”

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confidence: 99%

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Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Ali

Abid

Ahmed

et al. 2023

J Chinese Chemical Soc

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Herein, we present an experimental and theoretical drug-drug interaction study between nitazoxanide (NTZ) and azithromycin (AZT) in an aqueous solution. Interaction was studied by using UV/Vis, fluorescence, attenuated total reflectance-fourier transform infrared (ATR-FTIR), and circular dichroism (CD) spectroscopy, while molecular docking studies were performed to establish the interaction computationally. A bright yellow color was observed when the two drugs interacted, giving a hyperchromic band at 420 nm. The rate of absorbance was linearly increased by increasing drug concentrations and in a time-dependent manner. Stability of the interaction complex (i.e., NTZ: AZT) was measured at variable temperatures (25-80 C), pH (5.0-10.0) and ionic strength (0.05-2.0 M NaCl), and not only proved stable but also retained antimicrobial potential with reduced cellular toxicity. Mole ratio and Job's method of continuous variations were used to establish the binding stoichiometry and found to be 2:1. The calculated binding constant (k b = 8,400 M À1 ) and Gibb's free energy (ΔG = À22.4 KJ/mol) also suggested an energetically favorable interaction. FTIR spectra of NTZ: AZT complex in comparison with two drugs alone revealed significant interaction which was nicely complemented by molecular docking studies. Interaction was also successfully demonstrated in presence of carrier protein HSA and by spiking the two drugs in real samples of human plasma and urine.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Ali

Abid

Ahmed

et al. 2023

J Chinese Chemical Soc

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“…It is indicated for amoebiasis, helminthiasis, giardiasis etc. 1 Ofloxacin [OFX] is 9-fluoro-2, 3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7 H-pyrido [1,2,3-de] [1,4] benzoxazine-6-carboxylic acid [ Fig.1(b)].…”

Section: Introductionmentioning

confidence: 99%

Rp-HPLC Method for Simultaneous Estimation of Nitazoxanide and Ofloxacin From Bulk and Tablet Dosage Form

Khairnar¹,

Chaudhari²

2018

IRJPS

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A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Nitazoxanide and Ofloxacin in tablet dosage form. The separation was carried out using a mobile phase containing acetonitrile, methanol and 0.4 M citric acid, (60:30:10, v/v/v). The column used was Thermo C 18 (4.6mm*250 mm, 5 µ) with flow rate of 1 mL / min using UV detection at 300 nm. The retention time of Nitazoxanide and ofloxacin was 3.071 min and 12.47 min, respectively. The described method was linear over a concentration range of 5-30 μg/mL (r 2 >0.999) for Nitazoxanide and 2-12 μg/mL (r 2 >0.998) for Ofloxacin. The mean % recovery was found to be 99.96 % for Nitazoxanide and 101.05 % for Ofloxacin. The limit of detection (LOD) for Nitazoxanide and Ofloxacin were found to be 0.3788 and 0.0929 μg/mL, respectively. Whereas the limit of quantification (LOQ) for Nitazoxanide and Ofloxacin was 1.1479 μg/mL and 0.2816 μg/mL, respectively. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise, accurate and cost effective which is useful for the routine determination of Nitazoxanide and Ofloxacin bulk drug and in its tablet dosage form.

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Development of a Validated Stability- Indicating HPTLC Method for Nitazoxanide in Pharmaceutical Formulation

Cited by 2 publications

References 6 publications

Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Rp-HPLC Method for Simultaneous Estimation of Nitazoxanide and Ofloxacin From Bulk and Tablet Dosage Form

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