Rp-HPLC Method for Simultaneous Estimation of Nitazoxanide and Ofloxacin From Bulk and Tablet Dosage Form

Abstract

A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Nitazoxanide and Ofloxacin in tablet dosage form. The separation was carried out using a mobile phase containing acetonitrile, methanol and 0.4 M citric acid, (60:30:10, v/v/v). The column used was Thermo C 18 (4.6mm*250 mm, 5 µ) with flow rate of 1 mL / min using UV detection at 300 nm. The retention time of Nitazoxanide and ofloxacin was 3.071 min and 12.47 min, respectively. The described method was linear over a concentration range of 5-30 μg/mL (r 2 >0.999) for Nitazoxanide and 2-12 μg/mL (r 2 >0.998) for Ofloxacin. The mean % recovery was found to be 99.96 % for Nitazoxanide and 101.05 % for Ofloxacin. The limit of detection (LOD) for Nitazoxanide and Ofloxacin were found to be 0.3788 and 0.0929 μg/mL, respectively. Whereas the limit of quantification (LOQ) for Nitazoxanide and Ofloxacin was 1.1479 μg/mL and 0.2816 μg/mL, respectively. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise, accurate and cost effective which is useful for the routine determination of Nitazoxanide and Ofloxacin bulk drug and in its tablet dosage form.