Development of a validated liquid chromatography method for the simultaneous determination of eight fat-soluble vitamins in biological fluids after solid-phase extraction

Chatzimichalakis, Pavlos F.; Samanidou, Victoria F.; Papadoyannis, Ioannis N.

doi:10.1016/j.jchromb.2004.03.009

Cited by 100 publications

(67 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The proposed method was found to be 15-1000 times more sensitive than HPLC-UV methods [19][20] method [28] and with sensitivity similar to LC-MS method [29]. However, the high cost and the need for technical experience make LC-MS less convenient for routine assay of vitamin K in plasma of RA patients.…”

Section: Validation Of the Proposed Methodsmentioning

confidence: 99%

“…To date, several publications were reported for the separation and determination of vitamins K in biological samples including high performance liquid chromatography (HPLC) with ultraviolet (UV) [19][20], fluorescence [21][22][23][24][25], electrochemical [26][27], chemiluminescence (CL) [28] and mass spectrometric detection [29]. In initial study to detect vitamin K homologues in plasma of RA patients, it was found that vitamin K concentrations were significantly lower in RA patients that could not be detected by many of the reported methods [19][20][21][22][23][24][25][26][27][28] while others remain expensive techniques for routine assay of vitamin K concentrations [29].…”

Section: Introductionmentioning

confidence: 99%

“…In initial study to detect vitamin K homologues in plasma of RA patients, it was found that vitamin K concentrations were significantly lower in RA patients that could not be detected by many of the reported methods [19][20][21][22][23][24][25][26][27][28] while others remain expensive techniques for routine assay of vitamin K concentrations [29]. Therefore, it is necessary to develop a highly sensitive and selective determination method designated for measurements of lower concentrations vitamin K homologues in the presence of any existed interferences from medications or plasma components.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Ahmed

Kishikawa

Ohyama

et al. 2011

Talanta

View full text Add to dashboard Cite

Vitamin K is a fat-soluble vitamin involved in blood coagulation and bone metabolism. The detection and monitoring of vitamin K homologues in rheumatoid arthritis (RA) patients is a challenging problem due to the smaller concentrations of vitamin K and the presence of several interfering medications. Therefore, this study aimed to develop a new highly sensitive and selective chemiluminescence (CL) method designated to quantify vitamin K homologues in plasma of RA patients selectively including phylloquinone (PK, vitamin K 1 ), menaquinone-4 (MK-4, vitamin K 2 ) and menaquinone-7 (MK-7, vitamin K 2 ). The method was based on the unique photochemical properties of vitamin K homologues that were exploited for selective luminol CL reaction. The correlation coefficients of 0.998 or more were obtained in the concentration ranges of 0.1-100 ng mL -1 vitamin K homologues. The detection limits were 0.03-0.1 ng mL -1 in human plasma for vitamin K homologues. The developed HPLC-CL system was successfully applied for selective determination of vitamin K homologues in plasma of RA patients. The developed method may provide a useful tool for monitoring vitamin K homologues in different clinical studies such as RA, osteoporosis and hepatocellular carcinoma in which vitamin K is intervented.

show abstract

Section: Validation Of the Proposed Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Ahmed

Kishikawa

Ohyama

et al. 2011

Talanta

View full text Add to dashboard Cite

show abstract

“…About 150 mg of seaweed lipids was refluxed with methanolic KOH under N 2 in a water bath for half an hour and then the fat soluble vitamins were extracted with petroleum ether, identified and quantified by high performance liquid chromatography (HPLC; Shimadzu LC 10AS) equipped with C18 Reversed Phase column and ultraviolet (UV) detector as per Chatzimichalakis et al (2004) using acetonitrile and methanol in the simple linear gradient system.…”

Section: Fat Soluble Vitamin Analysismentioning

confidence: 99%

Nutritional profiling of the edible seaweeds Gracilaria edulis, Ulva lactuca and Sargassum sp.

Debbarama

Rao

Murthy³

et al. 2016

Indian J. Fish.

View full text Add to dashboard Cite

In the present study, nutritional composition of the edible seaweeds, Gracilaria edulis (red seaweed), Ulva lactuca (green seaweed) and Sargassum sp. (brown seaweed) were evaluated. ). Palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2, ω-6) were the most abundant saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA), respectively. Fatty acid profile also revealed that small quantity of docosahexaenoic acid (DHA) (C22:6, ω-3) ranging from 0.064 to 0.494% was present in all the three seaweed species but eicosapentaenoic acid (EPA) (C20:5, ω-3) was present only in Sargassum sp. at a concentration of 0.583%. G. edulis had higher vitamin D2 (2.590 mg 100 g -1 ), vitamin E (1.017 mg 100 g -1 ) and vitamin K1 (0.714 mg 100 g -1 ) than Sargassum sp. and U. lactuca. The results clearly indicates that these three seaweeds can be considered as a good source of dietary fibre, protein, minerals and vitamins and can be used for fortifying foods or as components of functional foods.

show abstract

“…Total lipids in the fish samples were extracted using a method developed by Folch et al (1957) and the fatty acids were classified using Gas Chromatography-mass spectrometry (Chatzimichalakis, Samanidou, & Papadoyannis, 2004). Each individual constituent was identified and quantified by comparing retention times and peaks with the standards.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Nutritive Profile of Sun Dried Fermented Mud Fish (Clarias Anguiliaris) and Tiger Fish (Hydrocynus Vittatus) Locally Known as Abil Alier Sold in Markets in South Sudan

Andrew¹,

Michael²,

Mandha³

2017

FSNS

View full text Add to dashboard Cite

show abstract

Development of a validated liquid chromatography method for the simultaneous determination of eight fat-soluble vitamins in biological fluids after solid-phase extraction

Cited by 100 publications

References 29 publications

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Nutritional profiling of the edible seaweeds Gracilaria edulis, Ulva lactuca and Sargassum sp.

Nutritive Profile of Sun Dried Fermented Mud Fish (Clarias Anguiliaris) and Tiger Fish (Hydrocynus Vittatus) Locally Known as Abil Alier Sold in Markets in South Sudan

Contact Info

Product

Resources

About