Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria

Abstract

The rapid and sensitive classification of bacteria is the first step of bacterial community researchand the treatment of infection. Herein, afluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among knownp robes with am inimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect aG ram-positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented researchtool for the study of dynamic bacterial communities and for clinical application.

One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens

“…3,6,15,16 In particular, achieving direct naked-eye visual identication of pathogens will be very benecial for rapid diagnosis at the point-of-care testing level. 17 Fluorescence is a promising visual tool for rapid and reliable identication of pathogens [18][19][20][21] because it exhibits more than a one thousand times improvement in sensitivity than colorimetry. 22 Gram-negative (GÀ) bacteria, G+ bacteria and fungi have different surface structures and chemical components (Scheme 1a), 6,23,24 which enable us to visually discriminate them using uorescence probes.…”

Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections

“…A number of FLI and/or PAI probes targeted to bacteria have been developed by using antibiotics (vancomycin 19,20 or teicoplanin 21 , specific to Gram-positive bacteria), Concanavalin (targeting bacterial cell-surface mannose) 22 , antibodies (targeting the immunodominant staphylococcal antigen A, specific to S. aureus) 23 , boronic-acid (targeting bacterial cell-surface glycoproteins, specific to Gram-positive bacteria) 24 , enzyme-activated nanoparticles (targeting gelatinase-expressing Gram-positive bacteria) 20 or through electrostatic and hydrophobic interactions (specific to Gram-positive bacteria) 25 . Preclinical evaluation of these probes showed promising results in FLI 19,[22][23][24] or PAI 20,21 of some bacterial infections. Unfortunately, targeting the bacterial cell wall potentially limits the amount of signaling agent taken up leading to lower sensitivity.…”

Visualizing semipermeability of the cell membrane using a pH-responsive ratiometric AIEgen

“…15 Thanks to the enthusiastic endeavors made by scientists, a lot of uorescent bioprobes have been developed for various applications. [16][17][18][19] Drugs with inherent uorescence make real-time in situ tracking of drug molecules in vivo or in vitro possible, which is of critical importance in pharmaceutical research. However, so far, uorescent drugs as probes for monitoring ion trapping have scarcely been reported in spite of their signicance in studying drug delivery and drug distribution in the body.…”