Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes

Gold nanoparticle-based electrochemical biosensors

“…Among the various strategies followed, a useful and simple way consists on the direct deposition of nanoparticles onto the electrode surface. For example, tyrosinase immobilization by cross-linking onto a GCE modified with electrodeposited gold nanoparticles was used to prepare a biosensor which showed a high activity towards various phenolic compounds [7]. Also, a glucose biosensor was prepared by covalent attachment of GOx to a gold nanoparticle monolayer-modified Au electrode [8].…”

Nanobiosensors for health care

“…In this context, biosensors based on the immobilization of enzymes on gold nanoparticles for the determination of hydrogen peroxide, nitrite, glucose and phenols (Tang & Jiang, 1998, Xiao et al, 2000, Gu et al, 2001, Liu & Ju, 2002, Jia et al, 2002, Liu et al, 2003b, Xiao et al, 2003, Carralero-Sanz et al, 2005 have been recently reported. The preparation of a tyrosinase biosensor based on the immobilization of the enzyme onto a glassy carbon electrode modified with electrodeposited gold nanoparticles (Tyr-nAu-GCE) was reported (Carralero-Sanz et al, 2005). The enzyme immobilized by cross-linking with glutaraldehyde retains a high bioactivity on this electrode material.…”

A field effect transistor (FET)-based immunosensor for detection of HbA1c and Hb

“…After the assembly of gold nanoparticles layer, a nanogold surface was obtained. It's well known that nanogold is of great benefit to biomolecular immobilization (Carralero et al 2005;Wu et al 2007;Qu et al 2009;Xu et al 2006;Riu et al 2006;Guo and Wang 2007). Thus, the antibody of HbA1c or Hb and BSA could be immobilized onto the electrode easily.…”