Development of a time-resolved immunofluorometric assay for quantitation of mucosal and systemic antibody responses

Hale, Martha L.; Campbell, Thomas A.; Campbell, Yvette; Fong, Steven E.; Stiles, Bradley G.

doi:10.1016/s0022-1759(01)00448-3

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…It also shows that the positive responses are extreme values of the corresponding age-specific distributions. The markedly stronger IgG fluorescence values are mainly due to the stronger fluorescence of Europium, which was used with anti-IgG detection antibodies, than of Samarium, which was used with anti-IgA antibodies [30].…”

Section: Resultsmentioning

confidence: 99%

Recent Diarrhea is Associated with Elevated Salivary IgG Responses to Cryptosporidium in Residents of an Eastern Massachusetts Community

et al. 2010

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Section: Resultsmentioning

confidence: 99%

Recent Diarrhea is Associated with Elevated Salivary IgG Responses to Cryptosporidium in Residents of an Eastern Massachusetts Community

et al. 2010

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“…Direct and indirect competitive TR-FIAs were compared in the measurement of methamphetamine in urine (Kimura et al 1999), showing that the indirect competitive format, using biotinylated antigen, was 100 Á/ 1000 times more sensitive than the direct, using Eu-labelled antigen. Hale et al (2001) compared direct with indirect TR-FIA in the measurement of serum immunoglobulins: Biotinylated antibody with Eu-streptavidin was found to be 100 times more sensitive than the same antibody conjugated to samarium and in sandwich TR-FIAs to determine human FSH and TSH (Suonpaa et al 1992), indirect TR-FIA using Eu-streptavidin was shown to be 2 Á/5 times more sensitive than direct TR-FIA. Hale et al (2001) also compared direct TR-FIA with ELISA, in the measurement of serum IgG and IgA, using the same antibodies conjugated to Eu, Sm or alkaline phosphatase (AP).…”

Section: Discussionmentioning

confidence: 99%

“…Hale et al (2001) compared direct with indirect TR-FIA in the measurement of serum immunoglobulins: Biotinylated antibody with Eu-streptavidin was found to be 100 times more sensitive than the same antibody conjugated to samarium and in sandwich TR-FIAs to determine human FSH and TSH (Suonpaa et al 1992), indirect TR-FIA using Eu-streptavidin was shown to be 2 Á/5 times more sensitive than direct TR-FIA. Hale et al (2001) also compared direct TR-FIA with ELISA, in the measurement of serum IgG and IgA, using the same antibodies conjugated to Eu, Sm or alkaline phosphatase (AP). The TR-FIAs were found to be respectively 10 and 37 times more sensitive than the corresponding ELISAs, but no comparison was made with HRPconjugated antibody.…”

Section: Discussionmentioning

confidence: 99%

A comparison of time-resolved fluoroimmunoassay and ELISA in the detection of casein in foodstuffs

Sletten

Løvberg

Moen

et al. 2005

Food and Agricultural Immunology

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With the aim of lowering the detection limit for casein in foods, three competitive assays are described: direct time-resolved fluoroimmunoassay (TR-FIA), using europium-conjugated antibody, indirect TR-FIA, using biotinylated antibody with europium-conjugated streptavidin and ELISA, using a HRP-conjugated secondary antibody. Food samples (instant potato, flour mix, packet soup, spice-mix) were analysed. Standard curve sensitivities in direct and indirect TR-FIAs did not differ significantly (p0/0.097), but both TR-FIAs were considerably less sensitive (both p B/0.0001) than ELISA (LOQs 1.3, 1.5 and B/1.0 mg kg (1 , respectively). The precision and working analyte range was similar in all three methods. Casein content measured in food products was comparable, using the three assays and rocket immunoelectrophoresis. The TR-FIA approach provided no improvement over the ELISA. All three assays allowed quantification of casein in foodstuffs in the order of 1 Á/1.5 mg kg (1 , providing a basis for more rigorous validation and collaborative testing.

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“…[18][19][20] Of particular concern to its applications is the study of coordination compounds in solution 21,22 and luminescent assays for biochemistry. 23, 24 After many experiments, the Eu-(II)-dipicolinate complex was employed to investigate the energy transfer mechanism for the HCO 4 -system. As shown in Figure 3, it was found that, by injecting Eu(III) (50 µL) into HCO 4 -solution, no CL emission was observed with our apparatus.…”

Section: On-line Preparation Of Hcomentioning

confidence: 99%

Chemiluminescence Energy Transfer Reaction for the On-Line Preparation of Peroxymonocarbonate and Eu(II)−Dipicolinate Complex

2006

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In this work, an on-line preparation of peroxymonocarbonate was formed innovatively, which offered a reliable intermediate for further investigation. The forming conditions of on-line peroxymonocarbonate ions were investigated in detail. Meanwhile, the energy transfer chemiluminescent reaction of peroxymonocarbonate and the Eu(II)-dipicolinate complex was studied. Through UV-visible absorption spectra, CL method, ESR spin-trapping technique, and mass spectrum experiments, it can be concluded that peroxymonocarbonate oxidizes Eu(II) to Eu(III), and simultaneously creates radicals. The bond rearrangement within radicals formed the singlet molecular oxygen. The energy originating from the singlet oxygen was accepted by the (Eu(III)dipic) -complex. The excited (Eu(III)*dipic)-ions underwent radiative deactivation and emitted the chemiluminescence. The peroxymonocarbonate system was a simple, inexpensive, and relatively nontoxic alternative to other oxidants, and it can be used in a mild, neutral-pH environment.

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Development of a time-resolved immunofluorometric assay for quantitation of mucosal and systemic antibody responses

Cited by 9 publications

References 24 publications

Recent Diarrhea is Associated with Elevated Salivary IgG Responses to Cryptosporidium in Residents of an Eastern Massachusetts Community

Recent Diarrhea is Associated with Elevated Salivary IgG Responses to Cryptosporidium in Residents of an Eastern Massachusetts Community

A comparison of time-resolved fluoroimmunoassay and ELISA in the detection of casein in foodstuffs

Chemiluminescence Energy Transfer Reaction for the On-Line Preparation of Peroxymonocarbonate and Eu(II)−Dipicolinate Complex

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