Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant

Chu, Chun-Yen; Liu, Chia‐Hui; Liou, Jhong-Jie; Lee, Jai-Wei; Cheng, Li-Ting

doi:10.1016/j.vaccine.2014.11.010

Cited by 42 publications

(36 citation statements)

References 35 publications

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“…As one of the main virulence factors of RA, OmpA has become important for RA serodetection, vaccine development, 19 and epidemiological analysis. A report showed that OmpA sequence variation correlated much better with serotyping than 16S-23S rRNA internal transcribed spacer region (ITS) sequencing, pulsed-¯eld gel electrophoresis (PFGE), or repetitive-sequence PCR (Rep-PCR).…”

Section: Discussionmentioning

confidence: 99%

“…16,17 The antisera were prepared by inoculating New Zealand White rabbits with the reference strain, which was a kind gift from Dr. ChaoFang Yu (Biologics Division, Animal Health Research Institute, Taiwan). 19 Cultured RA isolates (on agar plates for 24 h) were suspended in 1.0 mL of phosphatebu®ered saline (PBS), boiled for 1 h and then centrifuged at 8000 rpm for 10 min. Agar gel consisting of 0.9% Nobel agar (Difco) and 8.5% NaCl (Merk) were poured into 9-cm dishes for GDPT.…”

Section: Gel-di®usion Precipitin Test For Serotypingmentioning

confidence: 99%

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SURVEY OF ARIEMERELLA ANATIPESTIFEROUTBREAK IN SOUTHERN TAIWAN DUCK FARMS

et al. 2017

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A septicemic outbreak in southern Taiwan duck farms in 2014 resulted in high mortality of ducklings. Samples from oral or cloacal sites of a®ected Muscovy and Pekin ducks were collected and the identity of the¯eld isolates was con¯rmed using Riemerella anatipestifer (RA) 16S rRNA and outer membrane protein A (OmpA)-speci¯c primers in polymerase chain reactions (PCR), with 15 isolates found positive for both 16S rRNA and OmpA. Detection of both the 16S rRNA and OmpA genes could be a rapid PCR test for RA. Serotyping of the isolates using gel-di®usion precipitin test identi¯ed serotypes 1, 4, 6, 17, and 19 while a number of isolates were unidenti¯able. Sequence analysis of the OmpA gene found high identity (99.0-99.7%) among isolates in Taiwan. These results indicate that RA remains as a signi¯cant cause of duck septicemic disease in southern Taiwan.

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Section: Discussionmentioning

confidence: 99%

Section: Gel-di®usion Precipitin Test For Serotypingmentioning

confidence: 99%

SURVEY OF ARIEMERELLA ANATIPESTIFEROUTBREAK IN SOUTHERN TAIWAN DUCK FARMS

et al. 2017

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“…The CD8+ cells did not show any significant change in proportion throughout the study and ranged between 3.30 ± 0.87% -5.855 ± 0.67%. There was significant increase in CD4 + T lymphocytes from day 14 post immunization in ducks immunized with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant (Chu et al, 2015).…”

Section: Analysis Of Cd4mentioning

confidence: 96%

“…In another study it was demonstrated that there was significant increase in IFN-γ level 7 day post vaccination with marek disease vaccine (Abdul-Careem et al, 2008). Chu et al, 2015 demonstrated that there is significant up regulation of Th1 cytokine IFN-γ followed by immunization with subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant.…”

Section: Analysis Of Cd4mentioning

confidence: 99%