SURVEY OF A<i>RIEMERELLA ANATIPESTIFER</i>OUTBREAK IN SOUTHERN TAIWAN DUCK FARMS

Phonvisay, May; Liou, Jhong-Jie; Cheng, Li-Ting; Chen, Yen-Ping; Wu, Heng; Liu, Chia‐Hui; Lee, Jai-Wei; Chu, Chun-Yen

doi:10.1142/s1682648516500013

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…In a previous study, it was showed that the overall morbidity and mortality of duck may vary and upto 75% mortality were recorded depending on the age and level of stress factors (Subramaniam et al, 2000;Crasta et al, 2002). The mortality rate in ducklings below 8 weeks of age was found to be higher than adult ducks (Yu et al, 2008, Phonvisay et al, 2017. Various factors predispose susceptible ducklings to the disease such as concurrent disease, adverse climate conditions and nutritional status (Vandamme et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Molecular Characterization of Riemerella anatipestifer from Domesticated Ducks of Assam, India

Doley

Das

Sharma

et al. 2021

IJAR

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Background: Riemerella anatipestifer (R. anatipestifer) is a gram negative, microaerophilic, non-motile, bipolar bacteria. High genetic diversity and molecular differentiation were reported among field isolates. Although the bacterium causes one of the most economically important duck diseases in the north-eastern region of India, little work has been done on isolation, identification and molecular characterization of the bacteria. Hence, the present investigation was undertaken with a view to characterize the R. anatipestifer isolates from ducks of Assam.Methods: Phenotypic and molecular identification of R. anatipestifer isolates from domesticated ducks of Assam, India were carried out during the period from February, 2019 to January 2020. A total of 624 samples (Ocular swab, throat swab, liver, spleen, kidney, brain, heart, lung) from ducks comprising of apparently healthy, ailing and dead ducks were collected from five districts of Assam, India were processed to isolate and identify the bacteria. The tentative identification of the bacteria was done based on phenotypic characteristics viz., colony morphology, growth characteristics and biochemical reactions. All the phenotypically positive isolates were further subjected to molecular identification based on PCR assay targeting 16S rRNA gene and ERIC sequence.Result: The bacteria could be isolated from different field samples. The highest percentage of the samples that yielded the bacteria are from brain (76%) followed by spleen (74%) of dead ducks and less number of ocular swab (33%) from apparently healthy ducks were found positive. Sequencing of the amplified product of the selected R. anatipestifer isolates targeting 16S rRNA gene revealed homology percentage of 96.5-100%. Further, sequences representing five geographical locations were submitted to NCBI gene bank. Phylogenetic studies of the isolates indicated that there is prevalence of at least two genetically different strains of R. anatipestifer in the study area. The study suggested that the R. anatipestifer infection is endemic in Assam causing varying rate of morbidity (39%) and mortality (53%) and molecular based confirmation is necessary besides phenotypic identification.

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Section: Resultsmentioning

confidence: 99%

Isolation and Molecular Characterization of Riemerella anatipestifer from Domesticated Ducks of Assam, India

Doley

Das

Sharma

et al. 2021

IJAR

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“…Poor sanitation, nutrient deficiency, adverse ( e.g . stressful) environments or climates, and concomitant diseases usually predispose fowl to outbreaks of R. anatipestifer infection, especially those under 8 weeks old (3, 26, 34). In a survey of samples of waterfowl in Taiwan between 2006 and 2007, the significant prevalence (84.4%) of R. anatipestifer in farms revealed the potential risk of an outbreak (4, 14).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and antibiogram of Riemerella anatipestifer isolated from waterfowl slaughterhouses in Taiwan

Chang

Chen

Wang

et al. 2019

Journal of Veterinary Research

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Introduction: Laryngeal swab samples collected from three waterfowl slaughterhouses in central Taiwan were cultured and suspected isolates of Riemerella anatipestifer were identified by API 20NE and 16S rDNA PCR. Material and Methods: Serum agglutination was used for serotyping, and antimicrobial susceptibility was tested. Results: Seventy-six R. anatipestifer isolates were detected, and the prevalences in the ducks and geese were 12.3% (46/375) and 8.0% (30/375), respectively. The positive isolation rates were 65.6% for all arriving waterfowl, 76.0% for birds in the holding area, 1.6% for defeathered carcasses, but zero for degummed carcasses. A PCR examination detected R. anatipestifer in the slaughtering area frequently. Serotype B was dominant in both duck (34.8%) and goose (46.7%) isolates, but the wide serotype distribution may very well impede vaccination development. All isolates were resistant to colistin, and 79.7% were resistant to more than three common antibiotics. Conclusion: The results proved that most ducks had encountered antibiotic-resistant R. anatipestifer in rearing, which suggests that the bacterium circulates in asymptomatic waterfowl. It is worth noting that most waterfowl farms were found to harbour R. anatipestifer, and contaminated slaughterhouses are a major risk factor in its spread. Effective prevention and containment measures should be established there to interrupt the transmission chain of R. anatipestifer.

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“…The predominance of nonserotypable R. anatipestifer strains was also found in the survey conducted in Southern Taiwan duck farms. Among 11 tested strains, 6 of them were nonserotypable, accounting for 54.54% ( Phonvisay et al , 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Identification, serotyping and antimicrobial susceptibility of Riemerella anatipestifer isolated from ducks in Vietnam

Vo¹,

Dang²,

Le³

et al. 2022

Open Vet J

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Background: Septicaemia caused by Riemerella anatipestifer (R. anatipestifer) is a serious problem in the duck industry worldwide, and it is currently one of the major concerns for duck farming in Vietnam. Aim: This study was conducted to identify the causative agent of septicemia in ducks in Vietnam. The antimicrobial susceptibility and serotypes of R. anatipestifer isolates were also determined to provide valuable information for disease treatment and vaccine development. Methods: R. anatipestifer was isolated using blood agar and chocolate agar media. The commercial API 20NE microtest system and partial nucleotide sequence analysis of the 16s rRNA were used to identify R. anatipestifer strains. Serotypes were determined by slide agglutination test using standard antisera against R. anatipestifer. The disc diffusion method was utilized to investigate the antimicrobial susceptibility of R. anatipestifer isolated strains. Results: A total of 408 samples were collected from ducks with typical symptoms of septicemia for R. anatipestifer isolation. Sixty-nine R. anatipestifer strains were identified. Serotyping results showed that 30 out of 69 bacterial strains were classified as serotypes 1, 6, 8, 10, and 20, with serotype 10 being the most prevalent. The antimicrobial susceptibility test revealed that 100% of the bacterial isolates were susceptible to Amoxicillin/clavulanic acid and Imipenem. In contrast, the majority of R. anatipestifer strains were resistant to Nalidixic acid (89.9%), Streptomycin (75.4%), and Norfloxacin (72.5%). Conclusion: This is the first ever report in term of identification, serotyping and antimicrobial susceptibility tests of R. anatipestifer causing septicaemia in ducks of Vietnam, providing useful scientific informations for treatment as well as vaccine development to control the disease.

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SURVEY OF ARIEMERELLA ANATIPESTIFEROUTBREAK IN SOUTHERN TAIWAN DUCK FARMS

Cited by 8 publications

References 20 publications

Isolation and Molecular Characterization of Riemerella anatipestifer from Domesticated Ducks of Assam, India

Isolation and Molecular Characterization of Riemerella anatipestifer from Domesticated Ducks of Assam, India

Epidemiology and antibiogram of Riemerella anatipestifer isolated from waterfowl slaughterhouses in Taiwan

Identification, serotyping and antimicrobial susceptibility of Riemerella anatipestifer isolated from ducks in Vietnam

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