Development of a spin assay for reserve bilirubin loading capacity of human serum.

Abstract

The capacity of human serum albumin to bind bilirubin is of paramount importance in the prevention of neurological damage due to hyperbilirubinemia in the neonate. Currently, there is no rapid, reliable method to determine the reserve bilirubin loading capacity of serum albumin. A method using electron spin resonance spectroscopy with a dianionic

Bilirubin Metabolism and its Disorders

“…116 Free bilirubin concentration may be roughly estimated as the product of serum bilirubin concentration, concentration of reserve bilirubin binding sites on albumin, and the association constant for bilirubin. 117 Competitive binding by a 14 C-labeled ligand, monoacetyl-4,4¢-diaminodiphenyl sulfone, or a spin-labeled ligand 1-N-(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)5-N-(1-aspartate)-2,4-dinitrobenzene 118 has been used to determine reserve binding capacity.…”

Magnetic resonance assays of haloperidol in human serum albumin

“…Most of the recent studies have been using analytical assays to map out the number of binding sites on the HSA. This approach uses a variety of the spectroscopic methods such as Ultra Violet (UV) (Sakurai and Tsuchiya, 2006) and electron spin resonance (Hsia et al, 1978). In order to study the binding of drug to HSA, we examined the effect with haloperidol upon the relaxation of the fluorine atom.…”