“…In a previous work, we reported the development of a monoclonal antibody specific to grouper (3D12 MAb) (Asensio et al, 2003). Since the new fish fillet markets provide cheap fish species not marketed before, such as meagre, Nile tilapia, sutchi catfish, amber jack, striped mullet, pink cusk-eel and kingklip, among others, which potentially could be used as substitutes for grouper, we have evaluated the specificity of the 3D12 MAb against these and other fish species (see Materials and methods section) by using an immunostick colorimetric ELISA.…”