Development of a species-specific sequence-characterized amplified region marker for roses

Riaz, Sadaf; Sadia, Bushra; Awan, Faisal Saeed; Khan, Iqrar Ahmad; Sadaqat, Hafeez Ahmad

doi:10.4238/2012.february.24.3

Cited by 6 publications

(2 citation statements)

References 32 publications

(28 reference statements)

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“…This band pattern was the same as the result in Figure 1; therefore, the SCAK16 583 marker designed in this study was proved to be a species-specific marker of R. multiflora. The conversion of RAPD markers into SCAR markers was first described by Paran and Michelmore (1993), and SCAR markers have been developed on several crops for hybrid identification, such as chili pepper (Jang et al, 2004), sugarcane (Wang et al, 2009), casuarina (Ghosh et al, 2011) and rose (Riaz et al, 2012). The SCAK16 583 marker obtained in this study also can be useful for identifying hybrids of R. multiflora at an early developmental stage of the progeny.…”

Section: Conversion Of Rapd Marker Into Scar Markermentioning

confidence: 75%

Development of Species-specific SCAR Markers for Identification of Rose Species, Rosa multiflora

Zhuang

Aoki

Kageyama

et al. 2013

J. Japan. Soc. Hort. Sci.

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It has been attempted to develop multiple resistant rootstock against rose root rot (Pythium helicoides Drechsler) and crown gall disease (Rhizobium radiobacter (Beijerinck and van Delden, 1902) Young et al., 2001) using tetraploid Rosa multiflora 'Matsushima No.3' and R. 'PEKcougel'. However, hybrid identification based on conventional morphological characteristics is difficult, and establishment of hybrid diagnosis by DNA markers is desired. In this study, we attempted to develop specific DNA markers of R. multiflora. Three hundred 10 mer random oligonucleotide primers were screened, and a unique polymorphic fragment in R. multiflora amplified by OPAK16 primer was identified. This fragment could distinguish between roses with cross-fertilization genealogy with R. multiflora and roses with no relationship with R. multiflora. This specific fragment was cloned, sequenced and converted into a sequence characterized amplified region (SCAR) marker-SCAK16, which can amplify a 583 base pair (bp) fragment only in roses with relatedness to R. multiflora. This result indicated that SCAK16 583 was a species-specific marker of R. multiflora that could be used in a marker-assisted breeding program for hybrid identification of R. multiflora.

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Section: Conversion Of Rapd Marker Into Scar Markermentioning

confidence: 75%

Development of Species-specific SCAR Markers for Identification of Rose Species, Rosa multiflora

Zhuang

Aoki

Kageyama

et al. 2013

J. Japan. Soc. Hort. Sci.

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“…Linde et al (2004) and Yan et al (2005) developed SCAR markers in roses by utilizing RFLP markers. Whitaker et al (2010) reported SCAR marker development for a specific race of tetraploid roses against black spot resistance, and Riaz et al (2012) reported SCAR marker development for R. centifolia. But, there are still no reports available on SCAR marker development from RAPD in R. bourboniana.…”

Section: Introductionmentioning

confidence: 99%

Identification and authentication of Rosa species through development of species-specific SCAR marker(s)

et al. 2014

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ABSTRACT. Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is why a reliable fingerprinting method was developed to overcome this problem. The development of molecular markers will enable the identification of Rosa species. In the present study, randomly amplified polymorphic DNA (RAPD) analysis was done on four Rosa species, Rosa gruss-an-teplitz (Surkha), Rosa bourboniana, Rosa centifolia, and Rosa damascena. A polymorphic RAPD fragment of 391 bp was detected in R. bourboniana, which was cloned, purified, sequenced, and used to design a pair of speciesspecific sequence-characterized amplified region (SCAR) primers (forward and reverse). These SCAR primers were used to amplify the specific regions of the rose genome. These PCR amplifications with Identification of Rosa species by SCAR marker development specific primers are less sensitive to reaction conditions, and due to their high reproducibility, these species-specific SCAR primers can be used for marker-assisted selection and identification of Rosa species.

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How Far Advanced is the DNA-Based Identification of the BELFRIT-List?

Novak

Ruzicka

Schmiderer

2017

Food Supplements Containing Botanicals: Benefits, Side Effects and Regulatory Aspects

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Development of a species-specific sequence-characterized amplified region marker for roses

Cited by 6 publications

References 32 publications

Development of Species-specific SCAR Markers for Identification of Rose Species, Rosa multiflora

Development of Species-specific SCAR Markers for Identification of Rose Species, Rosa multiflora

Identification and authentication of Rosa species through development of species-specific SCAR marker(s)

How Far Advanced is the DNA-Based Identification of the BELFRIT-List?

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