Development of a SPE-LC/MS/MS method for simultaneous quantification of baicalein, wogonin, oroxylin A and their glucuronides baicalin, wogonoside and oroxyloside in rats and its application to brain uptake and plasma pharmacokinetic studies

Baicalein – main active flavonoid from Scutellaria baicalensis – voltammetric sensing in human samples using boron doped diamond electrode

“…It can also chelate with some metal cations, including copper(II), cobalt(II), nickel(II), and zinc(II), to form stable complex with antibacterial and scavenging superoxide radical properties [10,11]. Baicalein was widely analyzed by introducing capillary electrophoresis [12,13], gas chromatography [14], thin layer chromatography (TLC) [14,15], ion-pair (IP) high performance liquid chromatography (HPLC) [16], reversed phase HPLC [17,18], HPLC coupled to mass spectrometry (MS) [19], high speed counter current chromatography (HSCCC) [20] and different analytical techniques [21][22][23][24][25]. To the best of our knowledge, there are only few papers in the literature dealing with electrochemical determination of baicalein using DNA Langmuir-Blodgett modified electrode glassy carbon electrode [26].…”

Herb–drug interactions between Scutellariae Radix and mefenamic acid: Simultaneous investigation of pharmacokinetics, anti-inflammatory effect and gastric damage in rats

“…The content (w/w) of the six bioactive marker compounds baicalin, oroxyloside, baicalein, wogonoside, wogonin and oroxylin A in this SR extract are 48.0%, 4.1%, 2.0%. 1.7%, 0.4% and 0.1%, respectively, as quantified by our LC-MS/MS method (Fong et al, 2014c). SR extract was prepared as a suspension (150 mg/g) in water containing 5% (w/w) propylene glycol.…”

“…Plasma obtained at all the time points was subjected to solidphase extraction followed by LC-MS/MS analysis according to the method described previously (Fong et al, 2014c). After simultaneous quantification of the 6 major components of SR extract, MEF and its glucuronidated and hydroxyl metabolites, the plasma concentration versus time profile of each analyte was plotted and analyzed by WinNolin (Pharsight Corporation, Mountain View, USA, Version 2.1).…”

“…Since both the herb and the drug can contribute to the overall pharmacodynamic outcome, it is important to monitor the changes in the pharmacokinetics of both the drugs and the herbal components. To address this issue, we developed a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous measurement of MEF, the major components of SR, and their metabolites in biological samples (Fong et al, 2013(Fong et al, , 2014c. Another commonly seen deficiency is the lack of concurrent monitoring of both the pharmacokinetic and pharmacodynamic outcomes in a single in vivo interaction study (Fong et al, 2014b).…”

Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

“…This can further explain the high recovery of all concentrations of wogonin from group 1 and potentiates our hypothesis of such samples being incompatible with the test samples. Analysis of previous methods of wogonin quantitation in plasma showed that changing the plasma matrix with organic solvents was widely conducted during standard sample preparations (7,8,10,(16)(17)(18)(19)21). It was performed through direct spiking of the working standard stock solutions of wogonin (with organic solvents) in ratios ranging between 10-50 % of the blank plasma volume while ignoring the consequences for drug solubility and sample integrity and hence their validity.…”