Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction

Xu, Hongwei; Wang, Jiawen; Liu, Yameng; Wang, Yangyang; Zhong, Xianchun; Li, Cuina; Wang, Kanglong; Xie, Cen

doi:10.1016/j.aca.2023.341039

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…Sample preparation was performed as described previously. 9,10 Non-targeted metabolomics were performed using an ultrahigh-performance liquid chromatography system (UPLC; Waters, Milford, MA, USA) equipped with a quadrupole time-of-flight mass spectrometer (Q/TOF; Waters). Plasma metabolites were https://doi.org/10.2147/JIR.S448959…”

Section: Untargeted Metabolomics Analysis Using Uplc-q/tof-msmentioning

confidence: 99%

Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

Wang,

Wu,

Zhao

et al. 2024

JIR

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Purpose Postoperative cognitive dysfunction (POCD) is a central nervous system complication that occurs after anesthesia, particularly among the elderly. However, the neurological pathogenesis of postoperative cognitive dysfunction remains unclear. The aim of this study was to evaluate the effects of sevoflurane exposure on serum metabolites and hippocampal gene expression in elderly patients and aging mice by metabolomics and transcriptomic analysis and to explore the pathogenesis of sevoflurane induced POCD. Patients and Methods Human serum samples from five patients over 60 years old were collected before sevoflurane anesthesia and 1 hour after anesthesia. Besides, mice aged at 12 months (n=6 per group) were anesthetized with sevoflurane for 2 hours or with sham procedure. Subsequently, serum and hippocampal tissues were harvested for analysis. Further investigation into the relationship between isatin and neuroinflammation was conducted using BV2 microglial cells. Results Sevoflurane anesthesia led to the activation of inflammatory pathways, an increased presence of hippocampal astrocytes and microglia, and elevated expression of neuroinflammatory cytokines. Comparative analysis identified 12 differential metabolites that exhibited changes in both human and mouse serum post-sevoflurane anesthesia. Notably, isatin levels were significantly decreased after anesthesia. Notably, isatin levels significantly decreased after anesthesia, a factor known to stimulate proliferation and proinflammatory gene expression in microglia—the pivotal cell type in inflammatory responses. Conclusion Sevoflurane-induced alterations in serum metabolites in both elderly patients and aging mice, subsequently contributing to increased inflammation in the hippocampus.

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Section: Untargeted Metabolomics Analysis Using Uplc-q/tof-msmentioning

confidence: 99%

Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

Wang,

Wu,

Zhao

et al. 2024

JIR

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“…Although the value of studying the intestinal metabolome is clear, no analytical method that can simultaneously quantify SCFAs, BAs, AAs, and sugars in fecal samples has been reported. Although a UPLC-Q/TOF-MS method speci c for the intestinal metabolome can be found in the literature, this method is not able to measure bile acids, which are deeply implicated in intestinal health [15]. The limitations arise from the distinct properties of each metabolic compound, such as polarity, structure, and molecular weight.…”

Section: Introductionmentioning

confidence: 99%

Development of a specialized method for simultaneous quantification of functional intestinal metabolites by GC/MS-based metabolomics

Funahashi,

Fukuda,

Lee

et al. 2024

Preprint

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Intestinal metabolites produced by gut microbes play a significant role in host health. Due to their different chemical structures, they are often analyzed using multiple analyzers and methods, such as gas chromatography/mass spectrometry (GC/MS) for SCFAs and liquid chromatography/mass spectrometry (LC/MS) for bile acids (BAs), amino acids (AAs), and sugars. In this study, we aimed to develop a specialized method for the simultaneous determination of important intestinal metabolites, specifically addressing the main issue of SCFA volatilization during the dry solidification process. We discovered that these compounds can all be measured in fecal samples by GC/MS after trimethylsilyl (TMS) derivatization despite the expected volatility of SCFAs. Validating the results using SCFA standards suggested that the fecal matrix exerts a stabilizing effect. This method enabled the simultaneous quantification of 65 metabolites. For further validation in a biological context, a mouse study showed that high-MAC and high-fat diets increased SCFAs and BAs in feces, respectively, and showed a negative correlation between Alistipes and sugars, all consistent with previous studies. As a result, we successfully developed a specialized simultaneous quantification method for SCFAs, BAs, AAs, AA derivatives, and sugars in fecal samples using GC/MS-based metabolomics in conjunction with a TMS derivatization pretreatment process.

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Ootheca mantidis mitigates renal fibrosis in mice by the suppression of apoptosis via increasing the gut microbe Akkermansia muciniphila and modulating glutamine metabolism

Wang,

Guo,

Zou

et al. 2023

Biomedicine & Pharmacotherapy

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Development of a simultaneous quantification method for the gut microbiota-derived core nutrient metabolome in mice and its application in studying host-microbiota interaction

Cited by 3 publications

References 55 publications

Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

Development of a specialized method for simultaneous quantification of functional intestinal metabolites by GC/MS-based metabolomics

Ootheca mantidis mitigates renal fibrosis in mice by the suppression of apoptosis via increasing the gut microbe Akkermansia muciniphila and modulating glutamine metabolism

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