Development of a simple, fluoroimmunoassay for paraquat

Coxon, Ruth; Rae, C.; Gallacher, Gerard; Landon, J.

doi:10.1016/0009-8981(88)90106-4

Cited by 28 publications

(7 citation statements)

References 12 publications

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“…Vários métodos para determinação do paraquat têm sido descritos na literatura, incluindo métodos colorimétricos (13) , cromatográficos (1,9,19,21,29,39,40,51,72) , eletroforéticos (71,74,76) e imunológicos (7,18,25,48) .…”

Section: Diagnóstico Toxicológico E Metodologias Disponíveisunclassified

“…Os métodos imunológicos podem ser utilizados para triagem através de imunoensaio enzimático (1) ; ou na quantificação do paraquat através de radioimunoensaio (RIA) em soro (25) ; plasma, urina e fluido do lavado broncoalveolar utilizando anticorpos monoclonais (7) ; por enzyme linked immunosorbent assay (ELISA) em plasma (48) e imunoensaios com fluorescência em plasma (18) . Segundo Proudfoot et al (56) , a sobrevida do intoxicado depende da concentração da substância no organismo e do tempo após a ingestão.…”

Section: Diagnóstico Toxicológico E Metodologias Disponíveisunclassified

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Aspectos gerais e diagnóstico clinicolaboratorial da intoxicação por paraquat

Schmitt¹,

Paniz²,

Grotto³

et al. 2006

J. Bras. Patol. Med. Lab.

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Section: Diagnóstico Toxicológico E Metodologias Disponíveisunclassified

Aspectos gerais e diagnóstico clinicolaboratorial da intoxicação por paraquat

Schmitt¹,

Paniz²,

Grotto³

et al. 2006

J. Bras. Patol. Med. Lab.

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“…The ELISA optimized by Koivunen et al () in combination with the urine pretreatment detected PQ levels as low as 2 µg/L in spiked urine samples. Other immunoassays, such as radioimmunoassay (Levitt, ; Fatori and Hunter, ; Bowles et al , ) and fluoroimmunoassay (Coxon et al , ) have also been reported for the quantification of PQ in biological matrices.…”

Section: Introductionmentioning

confidence: 99%

Quantification of paraquat in postmortem samples by gas chromatography–ion trap mass spectrometry and review of the literature

Moreira

Pinho

Baltazar

et al. 2011

Biomedical Chromatography

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Paraquat (PQ) is an herbicide implicated in numerous fatalities, mainly caused by voluntary ingestion. Several methods have been used to quantify PQ in plasma and urine samples of intoxicated humans as a predictor of clinical outcome. There is no validated method for the analysis of PQ in postmortem samples. Therefore, the aim of this study was to develop an analytical method, using gas chromatography-ion trap mass spectrometry (GC-IT/MS) after solid-phase extraction, to quantify PQ in postmortem samples, namely in whole blood, urine, liver, lung and kidney, to cover the routes of distribution, accumulation and elimination of PQ. The method proved to be selective as there were no interferences of endogenous compounds with the same retention time as PQ and ethyl paraquat (internal standard). The regression analysis for PQ was linear in the range 0-10 µg/mL. The detection limits ranged from 0.0076 µg/mL for urine to 0.047 µg/mL for whole blood, and the recoveries were suitable for forensic analysis. The proposed GC-IT/MS method provided an accurate and simple assay with adequate precision and recovery for the quantification of PQ in postmortem samples. The proof of applicability was performed in two fatal PQ intoxications. A review of the analytical methods for the determination of quaternary ammonium herbicides is also provided for a better understanding of the presently available techniques.

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“…These instances have given rise to the need for a rapid sensitive assay to detect PQ in agricultural land and extracts of agricultural products. To date, many methods, which mainly include: gas chromatography (GC) [7], high-performance liquid chromatography (HPLC) [8], gas chromatographymass spectrometry (GC-MS) [9], liquid chromatography-mass spectrometry (LC-MS) [10], capillary electrophoresis [11], immunoassays [12][13][14][15][16][17][18][19], have been applied to detect residual PQ. GC, HPLC, GC-MS, LC-MS, and capillary electrophoresis technology have high sensitivity and accuracy.…”

Section: Introductionmentioning

confidence: 99%

Selection of an artificial paraquat‐specific binding protein from a ribosome display library based on a lipocalin scaffold

Liu

Ning³

et al. 2020

Biotechnology and Applied Biochemistry

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In this study, an anticalin that could specifically bind paraquat (PQ), a quaternary nitrogen herbicide, as a new set of engineered receptor protein with antibody-like properties was generated to detect PQ concentration. To this end, a native and random library was constructed and engineered to allow in vitro transcription and translation using an Escherichia coli lysate system. Meanwhile, a PQ derivative that carries an active aliphatic carboxylate group at the end of an aliphatic spacer arm was synthesized. Then, this compound was coupled covalently to the carrier protein bovine serum albumin/ovalbumin and amino-functionalized paramagnetic beads. Alternating selection in solution and immobilization in microtiter wells were used to pan mRNA-ribosome-antibody complexes. After several rounds of ribosome display, three variants were selected from a random library of the bilin-binding protein. The variants that could bind complex PQ with high affinity and exhibit IC 50 values as low as 14.039 ± 0.970 ng/mL were identified. Moreover, the limits of detection reached 0.083 ± 0.011 ng/mL. Our data suggest that the generation of anticalins may provide a promising alternative to recombinant antibody fragments to create a stable receptor protein against hapten with bioanalytical relevance.

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Development of a simple, fluoroimmunoassay for paraquat

Cited by 28 publications

References 12 publications

Aspectos gerais e diagnóstico clinicolaboratorial da intoxicação por paraquat

Aspectos gerais e diagnóstico clinicolaboratorial da intoxicação por paraquat

Quantification of paraquat in postmortem samples by gas chromatography–ion trap mass spectrometry and review of the literature

Selection of an artificial paraquat‐specific binding protein from a ribosome display library based on a lipocalin scaffold

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