Development of a simple and low cost microbioreactor for high-throughput bioprocessing

Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10‐129 in a microfluidic bioreactor

“…The surface tension of the cell‐free supernatant medium was measured at different time points using a Tensiometer (Kruss Digital Tensiometer, Model K9) by following the method described by Rahman et al [17].…”

“…The concentration of rhamnolipid in the sample was estimated by the orcinol method [17]. A 333 μl portion of the filtered supernatant was extracted twice with 1 ml of diethyl ether.…”

“…The samples were heated at 80 °C in a water bath for 30 min and cooled for 15 min at room temperature and the attenuance was measured at 420 nm. The rhamnolipid concentration was quantified from the standard L ‐rhamnose calibration curve between 0 and 50 μg/ml and the results were expressed as RE (rhamnose equivalents; mg/ml) by multiplying rhamnose values by a coefficient of 3.0 for monorhamnolipids (RL1) and 1.95 for dirhamnolipids (RL2), obtained from the correlation of pure rhamnolipids/rhamnose [17,19,20].…”

Microfluidic Bioreactors for Cell Culturing: A Review

“…Batch feed bioreactors represent a semi-closed system with static culture conditions. The majority of microbioreactors reported in the literature for microbial fermentations such as those reported by [34][35][36][37] are operated as batch fed mode. Batch bioreactor systems provide a number of advantages, including minimised contamination (due to short times of growth) and higher raw material conversion levels, resulting from a controlled growth period [38].…”

“…Microbioreactors platforms integrated with highly sensitive detection systems to monitor key variables (pH, dissolved oxygen and biomass) have been applied to screen and optimise conditions for high-throughput fermentation processes. During the last decade, several designs of such microbioreactors have been demonstrated for high-throuput bioprocessing [27,35,37,58,73,180]. The performance of these microbioreactors compares favourably with their conventional macroscale counterparts in terms of the measurement profiles of key physicochemical variables (pH, dissolved oxygen and optical density).…”