Development of a Sequence-tagged Site for the RAPD Marker Linked to Leaf Spot Resistance in Olive

Abstract

<jats:p>A sequence-tagged site (STS) was developed to identify a genetic marker linked to resistance to olive leaf spot caused by the pathogen, <jats:italic>Spilocea oleaginea</jats:italic> (Cast) (syn. <jats:italic>Cycloconium oleaginum</jats:italic> Cast.). The STS was based on a randomly amplified polymorphic DNA (RAPD) marker of about 780 base pairs (bp) linked to olive leaf spot resistance. Several primer pairs were developed to flank the sequence, and one pair produced the expected polymorphism between resistant and susceptible individuals tested, and was used as an STS marker. This primer pair was tested against parents and 34 individuals from a population segregating for resistance to olive leaf spot, and 12 commercial olive (<jats:italic>Olea europaea</jats:italic> L.) cultivars showing various levels of resistance to the disease. The STS marker was present in 71.4% of the parents and progeny that were designated as resistant, and was absent in 87% of the parents and progeny showing susceptibility. These primers were also able to distinguish cultivars such as `Koroneiki' and `Leccino', that are reported to show resistance to olive leaf spot, from `Barouni' and `Mission', that are reported to be susceptible. This is the first report of a STS marker for olive, and its use will assist greatly in screening olive progeny for resistance to leaf spot in breeding programs.</jats:p>

Olive leaf spot caused by Venturia oleaginea: An updated review

“…As a result, only very few resistant genotypes have been produced ( Lavee et al., 1999 ; Lavee et al., 2004 ). Further genomic ( Guerin et al., 2002 ; Mekuria et al., 2002 ) and transcriptomic ( Benitez et al., 2005 ) studies will be fundamental to identify genetic traits useful to assist breeding programs for OLS resistance.…”

Construction of a Genetic Linkage Map for the Olive Based on AFLP and SSR Markers

“…We specifi cally used the SCAR primer pair G7 developed by Mekuria et al (2002) since the authors assumed that it is linked to olive tree resistance to Spilocea oleagina L. The PCR reaction was performed as described previously for RAPD analysis with the following conditions: an initial denaturation at 95°C for 3 min followed by 34 cycles of 95°C for 30 s, 55°C for 45 s, and 72°C for 1 min with a fi nal extension step at 72°C for 10 min.…”

“…Using seven ISSRs and two RAPD primer pairs, we obtained 35 markers in the hybrid population: 15 segregating in PM, 14 in PL, and six in both parents (Table 3). The SCAR marker G7-700, considered as linked to peacock disease resistance (Mekuria et al, 2002), was present in both parents, and segregated in the hybrid population (present in 73 and absent in 67 progenies). Therefore, this marker was considered as a dominant marker and was not used during parental map construction but only in the consensus map.…”