Development of a sequence-specific PCR marker linked to the gene “pauper” conferring low-alkaloids in white lupin (Lupinus albus L.) for marker assisted selection

Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (<i>Lupinus albus</i> L.) Represent a Unique Genepool

“…These genotypes were important because they included the parents of the mapping population used to produce the L. albus linkage maps [22] [25], to locate the loci for low seed-alkaloid content (pauper) [34], and to develop PCR markers for resistance to anthracnose [35] and phomopsis [31]. In addition, they included parents of other mapping populations made for use in research to identify markers for loci controlling Pleiochaeta Root Rot resistance, and low seed alkaloid content locus exiguus [6].…”

Quantitative and qualitative analysis of alkaloids composition in the seeds of a white lupin (Lupinus albus L.) collection

“…At least five loci controlling alkaloid content are present in L. albus (Harrison and Williams 1982) and the one with pauper gene is the most effective in reducing alkaloid content and thus most frequently used in breeding (Lin et al 2009). Cultivars possessing pauper gene contain 0.02-0.05% alkaloids of the seed DW (Harrison and Williams 1982;Ś więcicki et al 2015) with following qualitative composition of major alkaloids (abundance [1%): lupanine-70%, albine-15%, 13-hydroxylupanine-8%, and multiflorine-3% (Wink et al 1995).…”

Evaluation of resistance to Phomopsis stem blight (caused by Diaporthe toxica) in Lupinus albus

“…In all cases, there were genotypic differences but there was not always agreement between experiments of resistance rankings due to Cowley et al (2010) the different environments in each experiment. Certain genotypes (most of the "P" lines in this study) that had been examined in glasshouse experiments could not be studied in the field because they contained alleles for high alkaloid content or alleles other than those used in commercial crops to condition the low-alkaloid trait (Lin et al 2009). These undesirable genes could have spread by pollen movement to neighbouring commercial L. albus crops .…”

“…Care should be exercised when using these lines in breeding with genotypes containing the pauper gene. Such crosses will result in high alkaloid progeny (Lin et al 2009) and may lead to bitter contamination in breeding populations and commercial crops .…”