Evaluation of resistance to Phomopsis stem blight (caused by Diaporthe toxica) in Lupinus albus

Cowley, Raymond; Ash, Gavin; Harper, John; Luckett, David J.

doi:10.1007/s10658-012-9942-3

Cited by 8 publications

(7 citation statements)

References 20 publications

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“…Therefore, the presented positions of D. toxica resistance loci (Table 4 ) should be considered as preliminary, tentative evaluations requiring further assessment. Several donors of Phomopsis stem blight resistance exist in WL collection, including wild accessions from Turkey (P27664), Syria (P27840, P28096) and Ethiopia (P28507, P27174) as well as cultivars from Australia (Andromeda, WALAB2008), France (Lublanc), Portugal (Madeira), Ukraine (Borki), and Germany (Ultra) 27 , 34 , 83 . Association mapping of 94 WL lines collected from 26 countries resulted in selection of numerous microarray-based DArT markers showing significant association with Phomopsis stem blight resistance, however, not a single marker was sequenced 83 .…”

Section: Resultsmentioning

confidence: 99%

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A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits

Książkiewicz

Yang

et al. 2017

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White lupin (Lupinus albus L.) is a valuable source of seed protein, carbohydrates and oil, but requires genetic improvement to attain its agronomic potential. This study aimed to (i) develop a new high-density consensus linkage map based on new, transcriptome-anchored markers; (ii) map four important agronomic traits, namely, vernalization requirement, seed alkaloid content, and resistance to anthracnose and Phomopsis stem blight; and, (iii) define regions of synteny between the L. albus and narrow-leafed lupin (L. angustifolius L.) genomes. Mapping of white lupin quantitative trait loci (QTLs) revealed polygenic control of vernalization responsiveness and anthracnose resistance, as well as a single locus regulating seed alkaloid content. We found high sequence collinearity between white and narrow-leafed lupin genomes. Interestingly, the white lupin QTLs did not correspond to previously mapped narrow-leafed lupin loci conferring vernalization independence, anthracnose resistance, low alkaloids and Phomopsis stem blight resistance, highlighting different genetic control of these traits. Our suite of allele-sequenced and PCR validated markers tagging these QTLs is immediately applicable for marker-assisted selection in white lupin breeding. The consensus map constitutes a platform for synteny-based gene cloning approaches and can support the forthcoming white lupin genome sequencing efforts.

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Section: Resultsmentioning

confidence: 99%

“…The fungus produces mycotoxins, phomopsins A and B, which cause lupinosis, a serious disease of sheep grazing contaminated stubble 26 . Several WL lines with moderate resistance were identified, including two Ethiopian accessions (P28507 and P27174) 27 .…”

Section: Introductionmentioning

confidence: 99%

A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits

Książkiewicz

Yang

et al. 2017

Sci Rep

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“…First, the infection of soybean by Diaporthe requires wounding. This was speculated by Cowley et al (2012), who used the spore injection inoculation method to evaluate resistance in Lupinus albus L. to D. toxica Williamson, Highet, Gams & Sivasithamparam. Cowley et al (2012) did not identify resistance to D. toxica among genotypes and they speculated that L. albus genotypes were susceptible to the pathogen only if the infection coincides with wounding of the genotypes from hail or insect feeding.…”

Section: Discussionmentioning

confidence: 99%

Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)

et al. 2019

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One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P < 0.001) and pathogen recovery (P < 0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate × method interaction affected disease severity (P < 0.05) and pathogen recovery (P < 0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate × method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.

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“…Shankar et al (2002a) postulated that stem and pod resistance in the L. angustifolius variety Tanjil is also controlled by different genes. Considerable genetic variation in the resistance to D. toxica exists within L. albus germplasm in leaves, stems and pods ( Cowley et al 2012a , 2012b , 2012c ), although knowledge is limited on the underlying genetics.…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs associated with resistance to Phomopsis pod blight (<i>Diaporthe toxica</i>) in <i>Lupinus albus</i>

et al. 2014

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Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively.

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Evaluation of resistance to Phomopsis stem blight (caused by Diaporthe toxica) in Lupinus albus

Cited by 8 publications

References 20 publications

A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits

A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits

Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)

Identification of QTLs associated with resistance to Phomopsis pod blight (<i>Diaporthe toxica</i>) in <i>Lupinus albus</i>

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