Development of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay (ic-ELISA) Based on the Monoclonal Antibody for the Detection of the Imidaclothiz Residue

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on monoclonal antibodies (MoAbs) for imidaclothiz was developed. The hapten of imidaclothiz was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the artificial antigens. MoAbs were obtained by immunizing BALB/c mice. Under the optimized conditions (10% methanol, 0.14 M Na(+), and pH 7.4), the half-maximal inhibition concentration (IC(50)) was 0.0875 ± 0.0034 mg/L and the limit of detection (IC(20)) was 0.0178 ± 0.0018 mg/L for imidaclothiz. There were no obvious cross-reactivities with most of the structural analogues of neonicotinoid insecticides, except imidacloprid. The recoveries of imidaclothiz in environmental and agricultural samples, including tap water, paddy water, soil, and cabbage, ranged from 80.43 to 113.83%, well within the requirements of residue detection. These results showed that this immunoassay could be used for the determination of imidaclothiz in environmental and agricultural samples.

Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

“…Haptens of parathion and imidacloprid were synthesized according to Liu et al 15 and Fang et al, 16 respectively. The structures of the haptens and pesticides are showed in Fig.…”

Development of an enzyme-linked immunosorbent assay for the simultaneous determination of parathion and imidacloprid

“…Haptens of parathion and imidacloprid were synthesized according to Liu et al, 9 Li and Li 10 and Fang et al, 11 respectively. The structures of the six haptens are showed in Fig.…”

Nanoplasmonic biochips for rapid label-free detection of imidacloprid pesticides with a smartphone

“…Although being sensitive and specific, these methods are high cost, time-consuming sample-preparation procedures and need highly trained technical personnel. In order to fulfill the requirement of rapid, simple, selective and sensitive detection, enzyme-linked immunosorbent assays (ELISAs) have been developed for detection of neonicotinoids in different matrices (Li and Li, 2000;Kim et al, 2004Kim et al, , 2006Watanabe et al, 2004Watanabe et al, , 2013Fang et al, 2011;Wang et al, 2012). However, repeated washing steps make the ELISA method difficult for one-step multiple assays.…”