Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses

Wan, Chunhe; Chen, Hongmei; Fu, Qiuling; Shi, Suhua; Fu, Guanghua; Cheng, Longfei; Chen, Cuiteng; Huang, Yu; Hu, Kaihui

doi:10.1292/jvms.15-0326

Cited by 11 publications

(15 citation statements)

References 12 publications

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“…Conventional PCR technology has been used for differentiation between MDPV and GPV, and the process includes restriction enzyme digestion, agarose gel electrophoresis, and DNA sequencing [12, 24]. Moreover, the PCR method for the specific detection of MDPV requires high precision primer design.…”

Section: Discussionmentioning

confidence: 99%

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

et al. 2018

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BackgroundMuscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV.ResultsThe specific primers and probe were designed based on the conserved regions within MDPVs, but there was a variation in GPVs of the nonstructural (NS) genes after genetic comparison. After the optimization of qPCR conditions, the detection limit of this qPCR assay was 29.7 copies/μl. The assay was highly specific for the detection of MDPV, and no cross-reactivity was observed with other non-targeted duck-derived pathogens. Intra- and inter-assay variability was less than 2.21%, means a high degree of repeatability. The diagnostic applicability of the qPCR assay was proven that MDPV-positive can be found in cloacal swabs samples, Muscovy duck embryos and newly hatched Muscovy ducklings.ConclusionsOur data provided incidents that MDPV could be possible vertically transmitted from breeder Muscovy ducks to Muscovy ducklings. The developed qPCR assay in the study could be a reliable and specific tool for epidemiological surveillance and pathogenesis studies of MDPV.

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Section: Discussionmentioning

confidence: 99%

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

et al. 2018

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“…The virus was harvested after three passages of infected Muscovy duck embryos. Five Muscovy duck embryos were used as control in order to make sure that no vertical transmission waterfowl parvoviruses were detected by the waterfowl parvoviruses universal primers described by us before [8] . Genomic nucleic acids were extracted using the Total DNA/RNA Isolation Kit (Omega Bio-Tek, GA, USA) according to the manufacture's instructions.…”

Section: Virus Isolation and Nucleic Acid Extractionmentioning

confidence: 99%

Çin’in Fujian Bölgesinde İzole Edilen Yeni Bir Ördek Parvovirus’unun Tüm Genom Sekansı

Wan¹,

Fu²,

Chen³

et al. 2016

Kafkas Univ Vet Fak Derg

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In the present study, we sequenced and analysed the complete genomes of a novel duck parvovirus (NM100) isolates derived from Muscovy ducks in Fujian, Southeast China. According to the phylogenetic analysis, based on the complete genome and VP1 gene showed that novel duck parvovirus strain NM100 belong to the MDPV clusters, whereas the VP3 gene showed that strain NM100 belong to the GPV clusters. Two putative genetic recombination events were detected using similarity plots analysis. These findings suggest that a novel duck parvovirus circulating in Muscovy duck flocks with recombination in nature, which enable us to understand the molecular characteristics and evolutionary diversity of waterfowl parvoviruses.

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“…Classical endemic and emerging viruses once outbreak in geese, such as goose parvovirus, avian influenza virus, avian Tembusu virus, avian paramyxovirus type 1, goose circovirus, Escherichia coli , Riemerella anatipestifer and Salmonella anatum spp . were excluded as the causative agent by PCR (RT-PCR) method [ 13 , 20 , 21 ].…”

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A novel group of avian <i>Avastrovirus</i> in domestic geese, China

Wan

Chen

Cheng

et al. 2018

The Journal of Veterinary Medical Science

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Using an ORF1b-based astrovirus-specfic reverse transcription (RT)-PCR assay, a novel astrovirus-like was detected from domestic geese in China. Pairwise comparisons and phylogenetic analyzes suggested that a novel group of goose astrovirus, different with previously known astroviruses in the genus Avastrovirus, was found circulating in geese. This study has expanded our understanding about the role of domestic waterfowls as reservoirs for diverse astroviruses.

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Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses

Cited by 11 publications

References 12 publications

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay

Çin’in Fujian Bölgesinde İzole Edilen Yeni Bir Ördek Parvovirus’unun Tüm Genom Sekansı

A novel group of avian <i>Avastrovirus</i> in domestic geese, China

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