A novel group of avian &lt;i&gt;Avastrovirus&lt;/i&gt; in domestic geese, China

Emerging Microbes & Infections

Ren

et al. 2018

Since the first isolation from human, astroviruses have been detected in many species. Wide host range and occasional cross-transmission of astrovirus pose a risk for zoonotic infection. Here, novel astroviruses were identified from goslings with recent epidemic gout disease in China. A virus, designated as GD, was efficiently isolated from a diseased gosling using LMH cells. Genome of GD amplified using 5′ and 3′ RACE was 7183nt in full length. Sequence analysis revealed the genome of GD was <60.8% homology with others deposited in Genbank. Moreover, GD could be neutralized by goose convalescent sera, and the gout associated symptom in goslings could be reproduced by GD infection. Our data demonstrated the goose astrovirus could be one of the causative agents of the ongoing gosling gout disease in China. The identification of the goose astrovirus not only diversified the astrovirus species, but also broadened the disease patterns caused by astroviruses.

Section: Discussionmentioning

confidence: 99%

An emerging novel goose astrovirus associated with gosling gout disease, China

Emerging Microbes & Infections

Ren

et al. 2018

“…The GoAstV representative isolate SD18, goose-origin Newcastle disease virus (NDV), goose-origin H9N2 subtype AIV, and duck AstV used in the experiment were isolated and identified by the SPF Chicken Research Center of Shandong (China). GoAstV393 was a positive plasmid for a 1b protein fragment cloned into PMD18-T ( 5 , 15 ). The viral simple RNA kit was from BioEr, Inc., and the qPCR kit and qPCR diluent were from TaKaRa Bio, Inc.…”

Section: Methodsmentioning

confidence: 99%

Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China

Yuan

Meng

et al. 2018

mSphere

Goose-origin astrovirus (GoAstV), as a newly emerging virus in 2017, is different from previously known astroviruses in the genus Avastrovirus. So far, few studies have focused on the novel virus. Considering the infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose GoAstV. Confirmed by clinical application, this method can quickly and accurately detect prevalent GoAstV. The assay is thus convenient for clinical operation and is applicable to the monitoring of GoAstV disease.

“…The virus was first identified in ducks in 1965 [ 1 ]. The AAstV genome length is 6.1–7.9kb, and it contains three open reading frames (ORFs: ORF1a, ORF1b and ORF2), a 5′ untranslated region (UTR), a 3′ UTR, and a poly (A) tail [ 2 ]. The family Astroviridae contains two genera, Mamastrovirus and Avastrovirus (AAstV), which consist of astroviruses that infect mammalian and avian species, respectively [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Surveillance and genetic diversity analysis of avian astrovirus in China

Jiang

et al. 2022

PLoS ONE

Avian astroviruses (AAstVs) have caused major problem for poultry breeding industries in China in recent years, and the goose gout caused by goose astrovirus has produced particularly great economic losses. To better understand the prevalence and genetic diversity of AAstVs in China, 1210 poultry samples collected from eight provinces were tested with reverse transcription-polymerase chain reaction (RT-PCR) to detect AAstV infections in different poultry populations. Then, Open reading frames 2 (ORF2) was amplified by specific primers, and the genetic evolution was analyzed. Our surveillance data demonstrate the diversity of AAstVs in China insofar as we detected 17 AAstVs, including seven chicken astroviruses (CAstVs), five avian nephritis viruses (ANVs), two goose astroviruses (GoAstVs), two duck astrovirus (DAstVs), and one new AAstV belonging to Avastrovirus Group 3. The positive rate of AAstV infection was 1.40%. Host analysis showed that CAstVs and ANVs were isolated from chickens, DAstVs and GoAstVs were isolated from ducks. Host-species-specific AAstVs infections were also identified in numerous samples collected at each stage of production. This study provides further evidence to better understand the epidemiology of AAstVs in different species of poultry in China.