Development of a reduction-sensitive diselenide-conjugated oligoethylenimine nanoparticulate system as a gene carrier

Gu, Zhongwei

doi:10.2147/ijn.s32961

Cited by 23 publications

(6 citation statements)

References 45 publications

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“…Previous studies showed that the bactericidal effects of honey were partly, if not entirely, due to its acidity, high osmolality, and H 2 O 2 content [41], [42]. These characteristics could also be cytotoxic to cells.…”

Section: Discussionmentioning

confidence: 99%

Tualang Honey Improves Human Corneal Epithelial Progenitor Cell Migration and Cellular Resistance to Oxidative Stress In Vitro

et al. 2014

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Stem cells with enhanced resistance to oxidative stress after in vitro expansion have been shown to have improved engraftment and regenerative capacities. Such cells can be generated by preconditioning them with exposure to an antioxidant. In this study we evaluated the effects of Tualang honey (TH), an antioxidant-containing honey, on human corneal epithelial progenitor (HCEP) cells in culture. Cytotoxicity, gene expression, migration, and cellular resistance to oxidative stress were evaluated. Immunofluorescence staining revealed that HCEP cells were holoclonal and expressed epithelial stem cell marker p63 without corneal cytokeratin 3. Cell viability remained unchanged after cells were cultured with 0.004, 0.04, and 0.4% TH in the medium, but it was significantly reduced when the concentration was increased to 3.33%. Cell migration, tested using scratch migration assay, was significantly enhanced when cells were cultured with TH at 0.04% and 0.4%. We also found that TH has hydrogen peroxide (H2O2) scavenging ability, although a trace level of H2O2 was detected in the honey in its native form. Preconditioning HCEP cells with 0.4% TH for 48 h showed better survival following H2O2-induced oxidative stress at 50 µM than untreated group, with a significantly lower number of dead cells (15.3±0.4%) were observed compared to the untreated population (20.5±0.9%, p<0.01). Both TH and ascorbic acid improved HCEP viability following induction of 100 µM H2O2, but the benefit was greater with TH treatment than with ascorbic acid. However, no significant advantage was demonstrated using 5-hydroxymethyl-2-furancarboxaldehyde, a compound that was found abundant in TH using GC/MS analysis. This suggests that the cellular anti-oxidative capacity in HCEP cells was augmented by native TH and was attributed to its antioxidant properties. In conclusion, TH possesses antioxidant properties and can improve cell migration and cellular resistance to oxidative stress in HCEP cells in vitro.

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Section: Discussionmentioning

confidence: 99%

Tualang Honey Improves Human Corneal Epithelial Progenitor Cell Migration and Cellular Resistance to Oxidative Stress In Vitro

et al. 2014

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“…There are materials that can respond to both oxidation and reduction triggers, which are incorporated into responsive polymers, e.g., diselenides with chemical structure like those of disulfides [29]. Diselenides allows for alternative triggers within nano-biotechnology applications [30].…”

Section: Redox-sensitive Polymersmentioning

confidence: 99%

Biomaterials for Drug Delivery: Sources, Classification, Synthesis, Processing, and Applications

Adeosun¹,

Ilomuanya²,

Gbenebor³

et al. 2020

Advanced Functional Materials

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A way to avoid or minimize the side effect that could result in drug delivery to cells with increased efficiency and performance in the health rehabilitation process is to use biocompatible and biodegradable drug carriers. These are essentially biomaterials that are metallic, ceramic, or polymeric in nature. The sources of these materials must be biological in its entire ramification. The classification, synthesis, processing, and the applications to which these materials are put are the essential components of having suitable target cell drug carriers. This chapter will be devoted to discussing biomaterials suitable as drug carrier for use in the health-related matters of rehabilitation.

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“…Di-(1-hydroxyethylene) diselenide was synthesized according to the reported method with some modifications [15]. In brief, 2.30 g (30 mmol) Se powder was added into a two-necked flask and dispersed in 15 mL water under nitrogen atmosphere.…”

Section: Synthesis Of Di-(1-hydroxyethylene) Diselenidementioning

confidence: 99%

Redox poly(ethylene glycol)-b-poly(l-lactide) micelles containing diselenide bonds for effective drug delivery

Zeng

Zhou

et al. 2015

J Mater Sci: Mater Med

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Bioreducible polymers have appeared as the ideal drug carriers for tumor therapy due to their properties of high stability in extracellular circulation and rapid drug release in intracellular reducing environment. Recently, the diselenide bond has emerged as a new reduction-sensitive linkage. In this work, the amphiphilic poly(ethylene glycol)-b-poly(L-lactide) containing diselenide bond has been synthesized and used to load anti-tumor drug, docetaxel (DTX), to form the redox micelles. It was found that the redox micelles showed a rapid response to glutataione (GSH), which resulted in a fast release of DTX in the presence of GSH. In contrast, <40 % of DTX was released from the micelles within 72 h under the normal condition (absence of GSH). The DTX-loaded redox micelles showed the significant inhibition effect to MCF-7 cells, and the cytotoxicity was dependent on the intracellular GSH concentrations. Moreover, considering the potentially clinical applications of the micelles through intravenous injection, the blood compatibility was also studied by the hemolysis analysis, activated partial thromboplastin time, prothrombin time and thromboelastography assays. These results confirmed that the redox micelles showed good blood safety, suggesting a potential application in tumor therapy.

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Development of a reduction-sensitive diselenide-conjugated oligoethylenimine nanoparticulate system as a gene carrier

Cited by 23 publications

References 45 publications

Tualang Honey Improves Human Corneal Epithelial Progenitor Cell Migration and Cellular Resistance to Oxidative Stress In Vitro

Tualang Honey Improves Human Corneal Epithelial Progenitor Cell Migration and Cellular Resistance to Oxidative Stress In Vitro

Biomaterials for Drug Delivery: Sources, Classification, Synthesis, Processing, and Applications

Redox poly(ethylene glycol)-b-poly(l-lactide) micelles containing diselenide bonds for effective drug delivery

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