Development of a Reconstructed Cornea from Collagen–Chondroitin Sulfate Foams and Human Cell Cultures

Vrana, Nihal Engin; Builles, Nicolas; Justin, V.; Bednarz, J; Pellegrini, Graziella; Ferrari, Barbara; Damour, O.; Hulmes, David J.S.; Hasırcı, Vasıf

doi:10.1167/iovs.07-1599

Cited by 78 publications

(49 citation statements)

References 44 publications

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“…59 For example, the presence of corneal fibroblasts is important for the health of the epithelial layer. 60 In vivo studies have demonstrated that transfer of respiratory epithelial cells to preformed fibrous capsules via fibrin glue improved their proliferation and differentiation. In fact, a fully differentiated, pseudostratified epithelium was produced, which was unlikely to be achieved by epithelial cell cultures alone.…”

Section: Functional Epithelium For Regenerative Medicinementioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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Section: Functional Epithelium For Regenerative Medicinementioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…This is made possible because of the recent advances in tissue engineering and organ culture. To date, several corneal tissue models have been produced in vitro (Griffith et al 1999;Doillon et al 2003;Alaminos et al 2006;Liu et al 2006;Vrana et al 2008). However, many difficulties are still present regarding corneal tissue engineering and its final clinical introduction (Porter et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…To solve this problem, several researchers have tried to tissue-engineer corneas using autologous cells cultured in various extracellular 3D matrices and synthetic polymers (Griffith et al 1999;Doillon et al 2003;Alaminos et al 2006;Liu et al 2006;Vrana et al 2008;Fagerholm et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of corneal stroma with decellularized porcine xenografts in a rabbit model

Yoeruek¹,

Bayyoud²,

Maurus³

et al. 2011

Acta Ophthalmologica

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ABSTRACT.Purpose: To evaluate the potential use of decellularized porcine stromal matrix (PSM) for reconstruction of corneal stroma in a rabbit model. Methods: Ten chinchilla bastard rabbit corneas were exposed to a circular half-thickness keratotomy with a 3.0 mm diameter at the central cornea. Porcine corneas were decellularized using hypotonic tris buffer, ethylene diamine tetraacetic acid (EDTA, 0.1%), aprotinin (10 K IU ⁄ ml) and 0.3% sodium dodecyl sulphate (SDS). The 3.0 mm in diameter decellularized corneal stromal xenograft was inserted into the pocket, and the incision was closed with four 10.0 nylon sutures. Clinical photographs were taken at day 1, day 7, day 30 and on a monthly basis for up to 6 months after transplantation. Six months after surgery, the rabbits were killed and eyes were enucleated. Haematoxylin-eosin (HE) and 4,6-diamidino-2-phenylindole (DAPI) staining were performed to confirm the complete removal of the corneal cells after decellularization of porcine corneas and repopulation with rabbit cells. Alcian blue staining was performed for analysing the structure of the extracellular matrix (ECM). Results: Efficient elimination of porcine cells was achieved by our decellularization protocol and confirmed via HE and DAPI stainings. Moreover, the major histoarchitectural ECM structure had been maintained as visualized by the alcian blue stain. Finally, the PSM was biocompatible with the host's epithelium evidenced as a regrowth covering the exposed xenograft. Conclusions: This novel technique of tissue engineering may provide one of many solutions to addressing anterior corneal pathological conditions in the face of a shortage of human corneal material.

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“…Briefly The monosaccharide compositions of CPFs were analyzed according to the following procedure [24], the CPFs samples (10 mg) were hydrolyzed with 10 ml of 2mol/L TFA at Constructs were lyophilized and stored in a desiccator at room temperature [25][26][27].…”

Section: Preparation and Analysis Of Ligusticum Wallichii Polysaccharidementioning

confidence: 99%

Development of a Cross-Linked Polysaccharide ofLigusticum wallichii– Squid Skin Collagen Scaffold Fabrication and Property Studies for Tissue-Engineering Applications

Zhang

Wang

et al. 2013

International Journal of Polymeric Materials and Polymeric Biom

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Porous composite scaffold with Ligusticum wallichii polysaccharide and squid skin collagen was synthesised and characterized. The composite scaffold showed good PBS retention ability, slow biodegradation rate, high mechanical strength and superb cell proliferation promoting ability, the PBS content value increased from 433.40% to 571.05%; the weight lost in 7 days decreased from 95.41% to 34.35%; the tensile strength of sample increased from 0.19Mpa to 0.81Mpa; in the 7 day, the relative growth rate of cell in composite(polysaccharide:collagen=5:5) is twice of that in pure collagen.While with the polysaccharide portion increaseed, the elongation at break were decreased from 93.09% to 9.60%.

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Development of a Reconstructed Cornea from Collagen–Chondroitin Sulfate Foams and Human Cell Cultures

Abstract: These results demonstrate that collagen-chondroitin sulfate scaffolds are good substrates for artificial cornea construction with good resilience, long-term culture capability, and handling properties.

Cited by 78 publications

References 44 publications

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Reconstruction of corneal stroma with decellularized porcine xenografts in a rabbit model

Development of a Cross-Linked Polysaccharide ofLigusticum wallichii– Squid Skin Collagen Scaffold Fabrication and Property Studies for Tissue-Engineering Applications

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