Development of a real time quantitative PCR assay for detection of porcine endogenous retrovirus

Argaw, Takele; Ritzhaupt, Armin; Wilson, Carolyn A.

doi:10.1016/s0166-0934(02)00140-4

Cited by 27 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay is also efficient since use of test plates specifically designed for the iCycler iQ ™ allowed assay of up to 384 samples/plate. The Weinstock, 2001 andMahlum et al, 2002), foot and mouth disease virus (Callahan et al, 2002), porcine endogenous retrovirus (Argaw et al, 2002), and rotavirus (Schwarz et al, 2002). Real-time RT-PCR assays were shown to be most efficient and sensitive for assay of clinical specimens infected with BVDV, when compared to gel-based RT-PCR assays, virus isolation tests, immunoperoxidase monolayer assays, and immunohistochemistry assays.…”

Section: Comparison Of Methodsmentioning

confidence: 99%

Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays

Achenbach

Topliff

Vassilev

et al. 2004

Journal of Virological Methods

View full text Add to dashboard Cite

Section: Comparison Of Methodsmentioning

confidence: 99%

Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays

Achenbach

Topliff

Vassilev

et al. 2004

Journal of Virological Methods

View full text Add to dashboard Cite

“…In order to measure provirus integration, PCR and real-time PCR methods have been developed (8,23,159,189,310), as well as Southern blot hybridization methods (3, 61), using PERV-specific primers and probes, respectively. In addition, a melting assay for estimation of the copy number of PERV in pigs has been developed (349).…”

Section: Pervsmentioning

confidence: 99%

Infection Barriers to Successful Xenotransplantation Focusing on Porcine Endogenous Retroviruses

2012

View full text Add to dashboard Cite

SUMMARY Xenotransplantation may be a solution to overcome the shortage of organs for the treatment of patients with organ failure, but it may be associated with the transmission of porcine microorganisms and the development of xenozoonoses. Whereas most microorganisms may be eliminated by pathogen-free breeding of the donor animals, porcine endogenous retroviruses (PERVs) cannot be eliminated, since these are integrated into the genomes of all pigs. Human-tropic PERV-A and -B are present in all pigs and are able to infect human cells. Infection of ecotropic PERV-C is limited to pig cells. PERVs may adapt to host cells by varying the number of LTR-binding transcription factor binding sites. Like all retroviruses, they may induce tumors and/or immunodeficiencies. To date, all experimental, preclinical, and clinical xenotransplantations using pig cells, tissues, and organs have not shown transmission of PERV. Highly sensitive and specific methods have been developed to analyze the PERV status of donor pigs and to monitor recipients for PERV infection. Strategies have been developed to prevent PERV transmission, including selection of PERV-C-negative, low-producer pigs, generation of an effective vaccine, selection of effective antiretrovirals, and generation of animals transgenic for a PERV-specific short hairpin RNA inhibiting PERV expression by RNA interference.

show abstract

“…Because of the shortage of human cells, porcine endogenous retroviruse (PERV), found in 1970's, was focused for its safety in xenotransplantation and treatments based on porcine tissues or cells as vitro infection of human cell lines HEK 293 was demonstrated in 1997 [4,[34][35][36][37] . Laboratory surveillance of PERV infection in pig xenograft recipients and the treatments is critical for determining the safety of pig xenotransplantation.…”

Section: Discussionmentioning

confidence: 99%

Detection of PERV by polymerase chain reaction and its safety in bioartificial liver support system

Wang

Wang²,

Liu³

et al. 2006

WJG

View full text Add to dashboard Cite

A I M : To e s t a b l i s h a m e t h o d d e t e c t i n g p o r c i n e endogenous retrovirus (PERV) inChina experimental minipigs and to evaluate the safety of PERV in three individuals treated with bioartificial liver support systems based on porcine hepatocytes. METHODS:Porcine hepatocytes were isolated with two-stage perfusion method, then cultured in the bioreactor, which is separated by a semipermeable membrane (0.2 μm) from the lumen through which the patients' blood plasma was circulated. After posthemoperfusion, patients' blood was obtained for screening. Additionally, samples of medium collected from both intraluminal and extraluminal compartments of the laboratory bioreactor and culture supernate in vitro was analyzed. The presence of viral sequences was estimated by polymerase chain reaction (PCR) and reverse transcriptase-polymerase chain reaction (RT-PCR). Finally, the infection of virus in the supernate of common culture was ascertained by exposure to the fetal liver cells.

show abstract

Development of a real time quantitative PCR assay for detection of porcine endogenous retrovirus

Cited by 27 publications

References 28 publications

Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays

Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays

Infection Barriers to Successful Xenotransplantation Focusing on Porcine Endogenous Retroviruses

Detection of PERV by polymerase chain reaction and its safety in bioartificial liver support system

Contact Info

Product

Resources

About