Development of a rainbow trout intestinal epithelial cell line and its response to lipopolysaccharide

Kawano, Atsushi; Haiduk, Christian; Schirmer, Kristin; Hanner, Robert; Lee, Lucy E. J.; Dixon, Brian; Bols, Niels C.

doi:10.1111/j.1365-2095.2010.00757.x

Cited by 122 publications

(120 citation statements)

References 64 publications

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“…By contrast, AP staining was absent in eelB cells from late-passage cultures whether they were in adhering neurospheres or in monolayer. Under the staining conditions of this study, most fish cell lines did not stain for AP (Xing et al 2009;Kawano et al 2011;Vo et al 2015a), although a zebrafish embryo cell line did (Xing et al 2008).…”

Section: Neurospheres Of Eelbmentioning

confidence: 83%

Development of a cell line from the American eel brain expressing endothelial cell properties

Bloch

Walsh

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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A cell line (eelB) was developed from the outgrowth of adherent cells from brain explants of the American eel, Anguilla rostrata (Lesueur). EelB cells have been grown routinely in L-15 with 10% fetal bovine serum (FBS), undergone over 100 passages, and cryopreserved successfully. The cells from late-passage cultures (>45) were polygonal, formed capillary-like structures (CLS) on Matrigel, and stained immunocytochemically for von Willebrand factor (vWF) and for three tight junction proteins, zonula occludens-1 (ZO-1), claudin 3, and claudin 5. These results suggest that eelB is an endothelial cell line, one of the few from fish and the first from the brain. Despite this, eelB did not respond to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) with the induction of CYP1A protein. The cells from early-passage cultures (<20) had more varied shapes and did not form CLS on Matrigel. Only cells from early-passage cultures formed in suspension three-dimensional aggregates that had some cells expressing alkaline phosphatase and nestin. These cells are thought to be neural stem cells and the aggregates neurospheres. The emergence of endothelial-like cells upon the continued subcultivation of cells from early-passage cultures that had neural stem cells has been described previously for mammals, but this is a first for teleosts. Remarkably, cells from all passage levels were stained strongly for senescence-associated β-galactosidase (SA β-Gal) activity.

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Section: Neurospheres Of Eelbmentioning

confidence: 83%

Development of a cell line from the American eel brain expressing endothelial cell properties

Bloch

Walsh

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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“…RTgutGC cells from rainbow trout intestinal epithelium were provided by Dr. Niels Bols (University of Waterloo, Canada) (Kawano et al, 2011). Cells were cultured and maintained in L-15 medium with glutamine (Life Technologies), supplemented with 10% FBS, penicillin (100 U/mL) and streptomycin (50 μg/mL).…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Schmitt

Wacyk

Morales‐Lange

et al. 2015

Developmental & Comparative Immunology

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“…From the data presented here, it seems likely that other cell lines isolated from ectothermic animals will store well in the enclosed fluidic biochip at temperatures in the low end of the organism's range. Other cell lines from the rainbow trout (RTgutGC (Kawano et al, 2010) and RTL-W1 (Lee et al, 1993)), which are derived from the intestine and liver, respectively, have been shown to survive for 9 months on the fluidic biochip at 6°C with no media replenishment, and still respond to toxicant challenges using ECIS (unpublished data, USACHER, 2012). This data supports the idea that different cells from the same organism (in this case the rainbow trout) have storage temperatures that are similar and in alignment with the whole organism's core temperature.…”

Section: Discussionmentioning

confidence: 99%

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

Curtis¹,

Collins²,

Gerlach³

et al. 2013

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Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, Using ECIS (electric cell-substrate impedance sensing) to monitor the impedance of vertebrate cell monolayers provides a sensitive measure of toxicity for a wide range of chemical toxicants. One major limitation to using a cell-based sensor for chemical toxicant detection in the field is the difficulty in maintaining cell viability over extended periods of time prior to use. This research was performed to identify cell lines suitable for ECIS-based toxicity sensing under field conditions. A variety of invertebrate and vertebrate cell lines were screened for their abilities to be stored for extended periods of time on an enclosed fluidic biochip with minimal maintenance. Three of the ten cell lines screened exhibited favorable portability characteristics on the biochips. Interestingly, all three cell lines were derived from ectothermic vertebrates, and the storage temperature that allowed long-term cell survival on the enclosed fluidic biochips was also at the lower end of reported body temperature for the organism. Future work with the ectothermic vertebrate cells will characterize their sensitivity to a wide range of chemical toxicants. Using ECIS (electric cell-substrate impedance sensing) to monitor the impedance of vertebrate cell monolayers provides a sensitive measure of toxicity for a wide range of chemical toxicants. One major limitation to using a cell-based sensor for chemical toxicant detection in the field is the difficulty in maintaining cell viability over extended periods of time prior to use. This research was performed to identify cell lines suitable for ECIS-based toxicity sensing under field conditions. A variety of invertebrate and vertebrate cell lines were screened for their abilities to be stored for extended periods of time on an enclosed fluidic biochip with minimal maintenance. Three of the ten cell lines screened exhibited favorable portability characteristics on the biochips. Interestingly, all three cell lines were derived from ectothermic vertebrates, and the storage temperature that allowed long-term cell survival on the enclosed fluidic biochips was also at the lower end of reported body temperature for the organism, suggesting that reduced cellular metabolism may be essential for longterm survival on the biochip. Future work with the ectothermic vertebrate cells will characterize their sensitivity to a wide range of chemical toxicants to determine if they are good candidates for use in a field portable toxicity sensor.

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Development of a rainbow trout intestinal epithelial cell line and its response to lipopolysaccharide

Cited by 122 publications

References 64 publications

Development of a cell line from the American eel brain expressing endothelial cell properties

Development of a cell line from the American eel brain expressing endothelial cell properties

Immunomodulatory effect of cathelicidins in response to a β-glucan in intestinal epithelial cells from rainbow trout

Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-Based Toxicity Sensor: Temperature Mediated Impacts on Long-Term Survival

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