Development of a quantum dot–based lateral flow immunoassay with high reaction consistency to total aflatoxins in botanical materials

Wu, Yuxiang; Yu, Binger; Cui, Ping; Yu, Tao; Guo-qing, Shi; Zhi-qiang, Shen

doi:10.1007/s00216-020-03123-4

Cited by 14 publications

(4 citation statements)

References 33 publications

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“…The mAb was prepared by the method previously described in our work [ 36 ], which was modified from the method described by Woychik et al [ 37 ]. In brief, DDB-BSA was emulsified with an equal volume of propolis adjuvant instead of traditional Freund’s adjuvant.…”

Section: Methodsmentioning

confidence: 99%

Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues

Fan

Chen

et al. 2022

Biosensors

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To produce a sensitive monoclonal antibody (mAb) for the simultaneous detection of carbofuran, benfuracarb, carbosulfan and 3-hydroxy-carbofuran, 2,3-dihydro-2,2-dimethyl-7-benzofuranmethanamine (DDB) was conjugated to bovine serum albumin (BSA) to prepare the immunogen DDB-BSA and mice were immunized. Coating antigens were prepared by conjugating DDB and 5-methoxy-2,3-dihydrobenzofuran-3-acetic acid (MDA) to BSA and ovalbumin (OVA), respectively. Furthermore, the effect of different antibody–antigen pairs on the sensitivity of ELISA and LFIA methods for the detection of carbofuran was investigated. After the immunization, a high-affinity mAb 13C8 was obtained. The ability of the coating antigen to compete with carbofuran for binding antibodies was found to be significantly different between ELISA and LFIA methods. With the antibody–antigen pair 13C8-MDA-OVA, the IC50 values of the ELISA and QD-LFIA methods for carbofuran were 0.18 ng/mL and 0.67 ng/mL, respectively. The cross-reactivity (CR) values of the two methods for benfuracarb, carbosulfan and 3-hydroxy-carbofuran ranged from 72.0% to 83.7%, while, for other carbamate pesticides, the CR values were less than 1%. The spiked recoveries of carbofuran in vegetables by the QD-LFIA method were 83–111%, with a coefficient of variation below 10%, and the test results of the actual samples were consistent with the HPLC-MS method. Overall, this study provides key materials for the development of immunoassays for carbofuran and its analogues, and the antibody–antigen pair selection strategy established in this study provides useful insights for the development of sensitive immunoassays for other compounds.

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Section: Methodsmentioning

confidence: 99%

Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues

Fan

Chen

et al. 2022

Biosensors

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“…The immunization schedule and antibody screening techniques also have an important effect on the quality of resultant antibodies. By using a rapid cell fusion technique, Wu et al [133] selected a cell line from 100,000 positive cell clones, which produced an mAb with similar recognition ability for AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2. Devi et al generated 10 hybridomas by immunizing AFB1-oxime-BSA to mice with an alternative immunization protocol [134].…”

Section: Antibodiesmentioning

confidence: 99%

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Wang

Zhang

Wang

et al. 2022

Toxins

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Mycotoxins are secondary metabolites produced by fungal species, which pose significant risk to humans and livestock. The mycotoxins which are produced from Aspergillus, Penicillium, and Fusarium are considered most important and therefore regulated in food- and feedstuffs. Analyses are predominantly performed by official laboratory methods in centralized labs by expert technicians. There is an urgent demand for new low-cost, easy-to-use, and portable analytical devices for rapid on-site determination. Most significant advances were realized in the field bioanalytical techniques based on molecular recognition. This review aims to discuss recent progress in the generation of native biomolecules and new bioinspired materials towards mycotoxins for the development of reliable bioreceptor-based analytical methods. After brief presentation of basic knowledge regarding characteristics of most important mycotoxins, the generation, benefits, and limitations of present and emerging biorecognition molecules, such as polyclonal (pAb), monoclonal (mAb), recombinant antibodies (rAb), aptamers, short peptides, and molecularly imprinted polymers (MIPs), are discussed. Hereinafter, the use of binders in different areas of application, including sample preparation, microplate- and tube-based assays, lateral flow devices, and biosensors, is highlighted. Special focus, on a global scale, is placed on commercial availability of single receptor molecules, test-kits, and biosensor platforms using multiplexed bead-based suspension assays and planar biochip arrays. Future outlook is given with special emphasis on new challenges, such as increasing use of rAb based on synthetic and naïve antibody libraries to renounce animal immunization, multiple-analyte test-kits and high-throughput multiplexing, and determination of masked mycotoxins, including stereoisomeric degradation products.

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“…Another example of application of QDs for improved detection in LFIA has been reported by Wu et al (2021). The aim was to improve the detection of AFB 1 in botanical materials by colloidal gold based LFIA, which is hampered by strong matrix effects.…”

Section: Single Mycotoxin or Single Mycotoxin Family Biosensorsmentioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2020-2021

Tittlemier¹,

Cramer

Dall’Asta

et al. 2022

WMJ

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This review summarises developments published in the period from mid-2020 to mid-2021 on the analysis of a number of diverse matrices for mycotoxins. Notable developments in all aspects of mycotoxin analysis, from sampling and quality assurance/quality control of analytical results, to the various detection and quantitation technologies ranging from single mycotoxin biosensors to comprehensive instrumental methods are presented and discussed. The summary and discussion of this past year’s developments in detection and quantitation technology covers chromatography with targeted or non-targeted high resolution mass spectrometry, tandem mass spectrometry, detection other than mass spectrometry, biosensors, as well as assays using alternatives to antibodies. This critical review aims to briefly present the most important recent developments and trends in mycotoxin determination, as well as to address limitations of the presented methodologies.

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Development of a quantum dot–based lateral flow immunoassay with high reaction consistency to total aflatoxins in botanical materials

Cited by 14 publications

References 33 publications

Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues

Production and Selection of Antibody–Antigen Pairs for the Development of Immunoenzyme Assay and Lateral Flow Immunoassay Methods for Carbofuran and Its Analogues

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

Developments in mycotoxin analysis: an update for 2020-2021

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