A noninvasive, highly
sensitive universal immunosensor platform
for protein-based biomarker detection is described in this Article.
A neutral charged sensing environment is constructed by an antibody
with an oppositely charged amino acid as surface charge neutralizer.
By adjusting the pH condition of the testing environment, this neutral
charged immunosensor (NCI) directly utilizes the electrostatic charges
of the analyte for quantification of circulating protein markers,
achieving a wide dynamic range covering through the whole picomole
level. Comparing with previous studies on electrostatic charges characterization,
this NCI demonstrates its capability to analyze not only the negatively
charged biomolecules but also positively charged analytes. We applied
this NCI for the detection of HE4 antigen with a detection limit at
2.5 pM and Tau antigen with a detection limit at 0.968 pM, demonstrating
the high-sensitivity property of this platform. Furthermore, this
NCI possesses a simple fabrication method (less than 2 h) and a short
testing turnaround time (less than 30 min), providing an excellent
potential for further clinical point-of-care applications.
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