Development of a protocol for the identification of tospoviruses and thrips species in individual thrips

Seepiban, Channarong; Charoenvilaisiri, Saengsoon; Kumpoosiri, Mallika; Bhunchoth, Anjana; Chatchawankanphanich, Orawan; Gajanandana, Oraprapai

doi:10.1016/j.jviromet.2015.06.017

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…Ribosomal RNA-ITS markers have been used for detection of S. dorsalis , T. palmi , F. tritici (Fitch), F. intonsa (Trybom), F. cephalica (Crawford), H. cahirensis (Trybom), Dendrothrips eremicola Priesner, Kakothrips pisivorus (Westwood), Hydatothrips kassimianus (Priesener), and Ceratothripoides claratris (Shumsher) [ 46 , 114 , 115 , 116 , 117 , 118 ]. ITS has been used as a natural choice of nuclear marker to substantiate mitochondrial marker-based identification and allow multi-locus phylogenetic analyses of F. occidentalis , F. intonsa , F. fusca (Hinds), T. tabaci , and Megalurothrips distalis (Karny) [ 72 , 119 , 120 ].…”

Section: Pcr-based Identification Of Thrips Using Molecular Markersmentioning

confidence: 99%

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Ghosh

Jangra

Dietzgen

et al. 2021

Insects

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Thrips are insect pests of economically important agricultural, horticultural, and forest crops. They cause damage by sucking plant sap and by transmitting several tospoviruses, ilarviruses, carmoviruses, sobemoviruses, and machlomoviruses. Accurate and timely identification is the key to successful management of thrips species. However, their small size, cryptic nature, presence of color and reproductive morphs, and intraspecies genetic variability make the identification of thrips species challenging. The use of molecular and electronic detection platforms has made thrips identification rapid, precise, sensitive, high throughput, and independent of developmental stages. Multi-locus phylogeny based on mitochondrial, nuclear, and other markers has resolved ambiguities in morphologically indistinguishable thrips species. Microsatellite, RFLP, RAPD, AFLP, and CAPS markers have helped to explain population structure, gene flow, and intraspecies heterogeneity. Recent techniques such as LAMP and RPA have been employed for sensitive and on-site identification of thrips. Artificial neural networks and high throughput diagnostics facilitate automated identification. This review also discusses the potential of pyrosequencing, microarrays, high throughput sequencing, and electronic sensors in delimiting thrips species.

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Section: Pcr-based Identification Of Thrips Using Molecular Markersmentioning

confidence: 99%

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Ghosh

Jangra

Dietzgen

et al. 2021

Insects

View full text Add to dashboard Cite

show abstract

An accurate, specific, sensitive, high-throughput method based on a microsphere immunoassay for multiplex detection of three viruses and bacterial fruit blotch bacterium in cucurbits

Charlermroj

Makornwattana

Himananto

et al. 2017

Journal of Virological Methods

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Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum

Alvarez-Diaz

Ortiz-Echeverry

Velásquez

2019

Journal of Virological Methods

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Development of a protocol for the identification of tospoviruses and thrips species in individual thrips

Cited by 4 publications

References 46 publications

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

An accurate, specific, sensitive, high-throughput method based on a microsphere immunoassay for multiplex detection of three viruses and bacterial fruit blotch bacterium in cucurbits

Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum

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