Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum

Alvarez-Diaz, Juan C.; Ortiz-Echeverry, Bianor A.; Velásquez, Nubia

doi:10.1016/j.jviromet.2018.12.007

Cited by 3 publications

(1 citation statement)

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“…Due to its contagious nature, the long latent period and the vegetative propagation of the chrysanthemum, the control measures of CSVd, listed as A2 quarantine pest by the European and Mediterranean Plant Protection Organisation [42], are based on the implementation of preventive strategies to avoid transmission by hands, tools and plant-to-plant contact. Other measures can be taken, such as obtaining viroid-free germplasm, removing sources of infectious materials and blocking viroid entry to the seedling production system by indexing phytosanitary programs supported by reliable diagnostic methods [8,31,43,44,45]. Moreover, strategies for managing stunt disease include natural or transgenic resistance [46,47,48], and anti-viroid agents [49].…”

Section: Introductionmentioning

confidence: 99%

Surveys in the Chrysanthemum Production Areas of Brazil and Colombia Reveal That Weeds Are Potential Reservoirs of Chrysanthemum Stunt Viroid

Gobatto

Oliveira

Franco

et al. 2019

Viruses

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The stunting disease, incited by chrysanthemum stunt viroid (CSVd), has become a serious problem in chrysanthemum production areas worldwide. Here we identified 46 weed species from chrysanthemum fields in two producing regions of the State of São Paulo, Brazil. The mechanical inoculation of these weeds with a Brazilian CSVd isolate revealed that this viroid was able to infect 17 of these species, in addition to chrysanthemum, tomato and potato. Plants of Oxalis latifolia and chrysanthemum naturally infected with CSVd were found in chrysanthemum fields in Colombia, which is the first CSVd report in that country. Therefore, weeds have the potential to act as reservoirs of CSVd in the field. These results are the first reports of experimental CSVd infection in the following species: Amaranthus viridis, Cardamine bonariensis, Chamaesyce hirta, Conyza bonariensis, Digitaria sanguinalis, Gomphrena globosa, Helianthus annuus, Lupinus polyphyllus, Mirabilis jalapa, Oxalis latifolia, Portulaca oleracea and Catharanthus roseus. The phylogenetic analyses of the CSVd variants identified herein showed three groups with Brazilian CSVd variants distributed in them all, which suggests that Brazilian CSVd isolates may have different origins through successive introductions of infected germplasm of chrysanthemum in Brazil.

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Section: Introductionmentioning

confidence: 99%

Surveys in the Chrysanthemum Production Areas of Brazil and Colombia Reveal That Weeds Are Potential Reservoirs of Chrysanthemum Stunt Viroid

Gobatto

Oliveira

Franco

et al. 2019

Viruses

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Microgram-grade concentration of chlorine dioxide induces one-step plant regeneration in chrysanthemum

Tian

Xie

Zhao

et al. 2022

In Vitro Cell.Dev.Biol.-Plant

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Development of a multiplex RT-PCR assay for simultaneous detection of Lily symptomless virus, Lily mottle virus, Cucumber mosaic virus, and Plantago asiatica mosaic virus in Lilies

Jun

2022

Virol J

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Background Viral pathogens causing significant economic losses in lilies (Lilium spp. and hybrids) include Lily symptomless virus (LSV), Lily mottle virus (LMoV), Cucumber mosaic virus (CMV), and Plantago asiatica mosaic virus (PlAMV). Rapid and efficient virus detection methods are pivotal to prevent the spread of these viruses. Results In this study, four specific primer pairs designed from conserved regions of genomic sequences of each virus were used to amplify a 116 bp product for LSV, a 247 bp product for LMoV, a 359 bp product for CMV, and a 525 bp product for PlAMV in a multiplex reverse transcription-polymerase chain reaction (multiplex RT-PCR). The amplified products were clearly separated by 2% agarose gel electrophoresis. The optimal reaction annealing temperature and cycle number were 53.8 °C and 35, respectively. The developed multiplex RT-PCR method was then used to test virus infections from lily samples collected from different regions of China. Conclusions An effective multiplex RT-PCR assay was established for the simultaneous detection and differentiation of LSV, LMoV, CMV, and PlAMV in lilies, which offers a useful tool for routine molecular diagnosis and epidemiological studies of these viruses.

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Duplex RT-PCR assay for simultaneous detection of TSWV and CSVd in chrysanthemum

Cited by 3 publications

References 45 publications

Surveys in the Chrysanthemum Production Areas of Brazil and Colombia Reveal That Weeds Are Potential Reservoirs of Chrysanthemum Stunt Viroid

Surveys in the Chrysanthemum Production Areas of Brazil and Colombia Reveal That Weeds Are Potential Reservoirs of Chrysanthemum Stunt Viroid

Microgram-grade concentration of chlorine dioxide induces one-step plant regeneration in chrysanthemum

Development of a multiplex RT-PCR assay for simultaneous detection of Lily symptomless virus, Lily mottle virus, Cucumber mosaic virus, and Plantago asiatica mosaic virus in Lilies

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