Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent

Hudson, James B.; Sharma, Manju; Vimalanathan, Selvarani

doi:10.1080/01919510902747969

Cited by 120 publications

(159 citation statements)

References 25 publications

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“…Most of the reported studies on plasma‐bio interaction are geared toward bacteria, while only a limited literature is available describing the inactivation of viruses and the mechanisms leading to virus inactivation . Ozone produced by commercially available ozonizers is found to be effective against viruses in hospital wards and operation theaters . However, RNS, rather than O 3 , is suggested to be mainly responsible for the inactivation of virus in solution by a surface DBD as suggested by Zimmermann et al Recently, Aboubakr et al reported on the inactivation effect of radio‐frequency (RF) plasma jets on feline calicivirus (FCV) and discussed the detailed chemical inactivation pathways consisting of two distinct chemistries inducing viral inactivation for a single plasma source: singlet oxygen/O 3 ‐based inactivation in the presence of O 2 and peroxynitrous acid‐based inactivation in the presence of air mediated by a mixture of H 2 O 2 and

N {normalO}_{2}^{-}

produced by the plasma …”

Section: Introductionmentioning

confidence: 99%

Reactive species responsible for the inactivation of feline calicivirus by a two‐dimensional array of integrated coaxial microhollow dielectric barrier discharges in air

Nayak¹,

Aboubakr

Goyal

et al. 2017

Plasma Processes & Polymers

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The use of low‐temperature plasmas for bio‐decontamination and sterilization has been gaining increased attention. In this study, a two‐dimensional array of integrated coaxial microhollow micro‐discharges generated in dry air at atmospheric pressure is used to treat metal surfaces (gas‐phase) and solution (liquid‐phase) contaminated with a known concentration of feline calicivirus (FCV). FCV acts as a surrogate for human norovirus, which is responsible for causing outbreaks of acute gastroenteritis in humans. The decontamination efficacy as well as the primary chemical pathways leading to virus inactivation in both the treatments are studied and compared. It is found that the humidity of the bio‐sample for gas‐phase treatment in dry air is required to achieve >5 log10 reduction in FCV titer within 3 min. The gas‐phase FCV inactivation is found to be due to a combination of ozone (O3) and reactive nitrogen species (RNS), most likely NOx. The liquid‐phase FCV inactivation mechanism is pH‐dependent and is primarily due to RNS, most likely acidified nitrites. O3 has a negligible effect on FCV suspended in solution. Previous studies performed in a batch reactor have shown that the inactivation pathways through O3 and RNS are mutually exclusive due to ozone poisoning at high NxOy concentrations. The present study employs a flow‐through system which avoids accumulation of reactive species and allows for the coexistence of NOx and O3 for the gas residence times used in this study, giving rise to these specific inactivation pathways.

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N {normalO}_{2}^{-}

produced by the plasma …”

Section: Introductionmentioning

confidence: 99%

Reactive species responsible for the inactivation of feline calicivirus by a two‐dimensional array of integrated coaxial microhollow dielectric barrier discharges in air

Nayak¹,

Aboubakr

Goyal

et al. 2017

Plasma Processes & Polymers

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show abstract

“…Because hydroxyl radicals have a higher oxidation potential than free oxygen atoms, the increased availability of water vapor may contribute to greater destruction of viral proteins and nucleic acids (Hudson et al 2009). In conclusion, when high levels of humidity are present, atmospheric ozone is a promising disinfection strategy decontaminating nonporous surfaces as it is effective against two surrogates for human norovirus.…”

Section: Results and Conclusionmentioning

confidence: 99%

“…Others have shown the response of FCV to atmospheric ozone disinfection (Hudson et al 2007(Hudson et al , 2009). However, because FCV has been reported to be more sensitive to several inactivating treatments (Cannon et al 2006;Park and Sobsey 2011), a side-by-side comparison of the response of two human norovirus surrogates was performed to better predict human norovirus disinfection upon exposure to atmospheric ozone in a high humidity environment.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Norovirus Surrogates after Exposure to Atmospheric Ozone

Cannon

Kotwal

Wang

2013

Ozone: Science & Engineering

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Human norovirus is the leading cause of epidemic gastroenteritis, especially in semiclosed settings such as daycares, nursing homes, hospitals, schools and on cruise ships. Outbreaks are often accompanied by contamination of environmental surfaces and commonly handled items. Surface disinfection of norovirus surrogates, feline calicivirus and murine norovirus, by 20 parts per million atmospheric ozone in a chamber maintaining 80% relative humidity was investigated. After treatment, neither virus could be detected on glass surfaces, but >5 log infectious virus was recovered from untreated controls. Ozone chambers used to decontaminate small, hand-contact items could be an important tool for controlling norovirus outbreaks.

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“…In order to eliminate the changes in the surrounding solution (H1N1). Previous results [29] indicated that there is about 1 log 10 decrease in virus infectivity due to drying. Starting from a titer of 10,000 PFUs, the virus titer was reduced to about 1000 PFUs after drying, before gas exposure.…”

Section: Nitrite Concentrati On Ph and Their Effect On Cell-free H1nmentioning

confidence: 92%

Gaseous nitric oxide reduces influenza infectivity in vitro

et al. 2013

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Gaseous nitric oxide (gNO) is an approved vasodilator drug for inhalation up to a maximum dose of 80 ppm. While gNO has been shown, in vitro, to be an effective antibacterial agent (at 160 ppm), NO-donor compounds have been shown to inhibit a variety of viruses at varying stages of replication. This research was done in order to determine whether gNO at 80 or 160 ppm possesses an antiviral effect on influenza viruses. Three strains of influenza (A and B) were exposed to gNO for up to 180 min, before and after infection of MDCK cells. In search for possible mechanism of antiviral action, Neuraminidase (NA) inhibition assay of H1N1 that was exposed to gNO was performed. Results show that when virions were exposed to gNO prior to infection a complete inhibition of infectivity was achieved for all three strains. Post infection exposure of influenza with gNO resulted in about 30% inhibition of infectivity. Further testing showed that when eliminating the pH effect by exposing a dried virus to gNO, 90% inhibition was found after 2h exposure. NA activity, of whole dried H1N1 virus, was found to be inhibited by gNO (80%). These results suggest that 80 and 160 ppm gNO have a time dependent antiviral effect on influenza strains of viruses during various stages of cellular infection, which are not due to concomitant changes in pH in the surrounding milieu. Viral NA inhibition by gNO was shown and may be responsible for this antiviral effect.

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Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent

Cited by 120 publications

References 25 publications

Reactive species responsible for the inactivation of feline calicivirus by a two‐dimensional array of integrated coaxial microhollow dielectric barrier discharges in air

Reactive species responsible for the inactivation of feline calicivirus by a two‐dimensional array of integrated coaxial microhollow dielectric barrier discharges in air

Inactivation of Norovirus Surrogates after Exposure to Atmospheric Ozone

Gaseous nitric oxide reduces influenza infectivity in vitro

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