Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies

Silva, A.I.; Mateus, Marı́lia

doi:10.1016/j.jbiotec.2008.12.004

Cited by 28 publications

(15 citation statements)

References 29 publications

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“…Thermoplastic materials that are currently used for cell immunoprotection are polyacrylonitrile and polyvinyl chloride copolymers (PAN/PVC or Amicon ; hollow fibers)(97, 113, 168, 169), polyurethane (PU) and polyurethane and polyvinyl pyrrolidone copolymers (PU/PVP) (macrocapsules)(170, 171), PU (membranes)(172), polysulfone (hollow fibers)(173, 174), AN69 renal dialysis membranes (69% acrylonitrile and 31% sodium methallyl sulphonate) modified by electrical discharges(175), polytetrafluoroethylene (PTFE) membranes(98, 99, 176, 177), dual porosity nylon membrane(178). ..…”

Section: Current Immunoprotective Membrane Designsmentioning

confidence: 99%

Membranes to achieve immunoprotection of transplanted islets

2014

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Transplantation of islet or beta cells is seen as the cure for type 1 diabetes since it allows physiological regulation of blood glucose levels without requiring any compliance from the patients. In order to circumvent the use of immunosuppressive drugs (and their side effects), semipermeable membranes have been developed to encapsulate and immunoprotect transplanted cells. This review presents the historical developments of immunoisolation and provides an update on the current research in this field. A particular emphasis is laid on the fabrication, characterization and performance of membranes developed for immunoisolation applications.

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Section: Current Immunoprotective Membrane Designsmentioning

confidence: 99%

Membranes to achieve immunoprotection of transplanted islets

2014

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“…It is possible to make fibers by spinning from a variety of materials suitable for cell culture, both biodegradable and non-biodegradable, for example; polycaprolactone (PCL) 27 , poly-L-lactide acid (PLLA) 28 , poly(lactic-co-glycolic acid) (PLGA) 29 , polysulfone (PSU) 12 and polyetheretherketone (PEEK)…”

Section: Discussionmentioning

confidence: 99%

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Storm

Sorrell

Shipley

et al. 2016

JoVE

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Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO 2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture. Video LinkThe video component of this article can be found at

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“…A wide array of biomaterials have been used since the very early chambers prototypes, such as PVC (polyvinyl chloride), PTFE (poly-tetra-ethylene) [20], or polysulfone [21], all proven to be poorly biocompatible, thus generating plenty of fibrotic tissue overgrowth leading to impairment of the chamber's diffusion properties. The idea of prevascularizing the device, using endothelial cells, to enhance the membrane's functional performance, has been associated with somehow increased tissue rejection rate [22].…”

Section: Macrodevicesmentioning

confidence: 99%

Immunoisolation of Pancreatic Islet Grafts with No Recipient’s Immunosuppression: Actual and Future Perspectives

Basta

Calafiore

2011

Curr Diab Rep

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In spite of steady and remarkable progress, islet transplantation in patients with type 1 diabetes mellitus (T1DM) continues to face two major bottlenecks: inadequate availability of human pancreatic donors and necessity to totally immunosuppress the graft recipients lifelong. Microencapsulation of the islet grafts within highly biocompatible and selective permeable biomembranes could obviate use of the immunosuppressants, while potentially offering the opportunity to use a wide array of insulin-producing cells, in active development, including xenogeneic pig islets. Although macrodevices and microcapsules, which essentially differ by size/configuration, and both serve for immunoisolation devices, have been used for many years with initial human applications, new products on development in both areas might open new perspectives for more focused use in patients with T1DM. Physical-chemical properties and material engineering of these devices are critically reviewed to assess where we actually stand and where the future expansion of these technologies may go.

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Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies

Cited by 28 publications

References 29 publications

Membranes to achieve immunoprotection of transplanted islets

Membranes to achieve immunoprotection of transplanted islets

Hollow Fiber Bioreactors for <em>In Vivo</em>-like Mammalian Tissue Culture

Immunoisolation of Pancreatic Islet Grafts with No Recipient’s Immunosuppression: Actual and Future Perspectives

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