Membranes to achieve immunoprotection of transplanted islets

Schweicher, Julien; Nyitray, Crystal; Desai, Tejal A.

doi:10.2741/4195

Cited by 77 publications

(69 citation statements)

References 229 publications

(271 reference statements)

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“…Macroencapsulation‐based devices developed as intravascular or extravascular implants have been used to treat metabolic and neurodegenerative disorders. A recent, comprehensive review on macroencapsulation devices has been published elsewhere …”

Section: Cell Immobilization: Conceptmentioning

confidence: 99%

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

2017

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The primary aim in tissue engineering is to repair, replace, and regenerate dysfunctional tissues to restore homeostasis. Cell delivery for repair and regeneration is gaining impetus with our understanding of constructing tissue‐like environments. However, the perpetual challenge is to identify innovative materials or re‐engineer natural materials to model cell‐specific tissue‐like 3D modules, which can seamlessly integrate and restore functions of the target organ. To devise an optimal functional microenvironment, it is essential to define how simple is complex enough to trigger tissue regeneration or restore cellular function. Here, the purposeful transition of cell immobilization from a cytoprotection point of view to that of a cell‐instructive approach is examined, with advances in the understanding of cell–material interactions in a 3D context, and with a view to further application of the knowledge for the development of newer and complex hierarchical tissue assemblies for better examination of cell behavior and offering customized cell‐based therapies for tissue engineering.

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Section: Cell Immobilization: Conceptmentioning

confidence: 99%

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

2017

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“…For instance, the immunoisolation of islet or beta cells with TiO 2 NT membranes could potentially lead to a treatment of type 1 diabetes 37 since the diffusion of IgG is largely hindered as compared to glucose and insulin.…”

Section: Resultsmentioning

confidence: 99%

Facile synthesis of robust free-standing TiO2 nanotubular membranes for biofiltration applications

Schweicher

Desai

2013

J Appl Electrochem

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Robust monodisperse nanoporous membranes have a wide range of biotechnological applications, but are often difficult or costly to fabricate. Here, a simple technique is reported to produce free-standing TiO2 nanotubular membranes with through-hole morphology. It consists in a 3-step anodization procedure carried out at room temperature on a Ti foil. The first anodization (1 h at 80 V) is used to pattern the surface of the metallic foil. Then, the second anodization (24 h at 80 V) produces the array of TiO2 nanotubes that will constitute the final membrane. A higher voltage anodization (3-5 minutes at 180 V) is finally applied to detach the TiO2 nanotubular layer from the underlying Ti foil. In order to completely remove the barrier layer that obstructs some pores of the membrane, the latter is etched 2 minutes in a buffered oxide etch solution. The overall process produces 60 μm-thick TiO2 nanotubular membranes with tube openings of 110 nm on one side and 73 nm on the other side. The through-hole morphology of these membranes has been verified by performing diffusion experiments with glucose, insulin and immunoglobulin G where in differences in diffusion rate are observed based on molecular weight. Such biocompatible TiO2 nanotubular membranes, with controlled pore size and morphology, have broad biotechnological and biomedical applications.

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“…In the last two decades, the concept of allo-transplantation has been studied in donor pancreatic islets encapsulated in an immuno-isolating device for treatment of Type 1 diabetes. 1,16,19,25 An immuno-isolating device creates a barrier between the implanted allogeneic cells or tissue and the host and eliminates the need for systemic immunosuppression. 19 The requirements from an efficient immuno-isolation device are two-fold.…”

Section: Introductionmentioning

confidence: 99%

“…1,16,19,25 An immuno-isolating device creates a barrier between the implanted allogeneic cells or tissue and the host and eliminates the need for systemic immunosuppression. 19 The requirements from an efficient immuno-isolation device are two-fold. First, it must provide a supportive environment for the implanted cells allowing sufficient diffusion of nutrients and oxygen.…”

Section: Introductionmentioning

confidence: 99%

Restoring Ovarian Endocrine Function with Encapsulated Ovarian Allograft in Immune Competent Mice

et al. 2016

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Premature ovarian insufficiency (POI) is a major complication of cytotoxic treatments due to extreme ovarian sensitivity to chemotherapy and radiation. In pediatric cancer patients modern therapy has improved the long-term survival to over 80% in the United States. However, these cancer survivors face long-term health problems related to treatment toxicity. In female cancer survivors POI leads to sterility, along with the consequences of estrogen deficiency such as premature osteopenia, muscle wasting, accelerated cardiovascular diseases and a vast array of other health and developmental problems. These long-lasting effects are particularly significant for young girls reaching puberty. As such, restoring ovarian endocrine function is paramount in this population. In the present study, we evaluated the feasibility of restoring ovarian endocrine function in ovariectomized mice by transplanting syngeneic and allogeneic ovarian tissue encapsulated in alginate capsules or TheraCyte®. Histological analysis of the implants retrieved after 7 and 30 days' post implantation showed follicular development up to the secondary and antral stages in both syngeneic and allogeneic implants. Implantation of syngeneic and allogeneic ovarian grafts encapsulated in TheraCyte devices restored ovarian endocrine function, which was confirmed by decreased serum FSH levels from 60 to 70 ng/mL in ovariectomized mice to 30–40 ng/mL 30 days after implantation. Absence of allo-MHC—specific IgG and IgM antibodies in the sera of implanted mice with allogeneic ovarian tissue encapsulated in TheraCyte indicate that the implants did not evoke an allo-immune response, while the allogeneic controls were rejected 21 days after implantation. Our results show that TheraCyte effectively isolates the graft from immune recognition but also supports follicular growth.

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Membranes to achieve immunoprotection of transplanted islets

Cited by 77 publications

References 229 publications

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

Toward Customized Extracellular Niche Engineering: Progress in Cell‐Entrapment Technologies

Facile synthesis of robust free-standing TiO2 nanotubular membranes for biofiltration applications

Restoring Ovarian Endocrine Function with Encapsulated Ovarian Allograft in Immune Competent Mice

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