Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene

Zarrin, Majid; Ganj, Farzaneh; Faramarzi, Sama

doi:10.3892/br.2016.783

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The occurrence of Fusarium spp. is currently identified using molecular markers and phylogenetic analysis mainly based on sequences of the transcription elongation factor gene TEF-1a [ 23 , 24 , 25 ]. In asparagus Fusarium species-specific primers for PCR analysis have been developed [ 26 , 27 ], and the genetic diversity analyses by PCR-denaturing gradient gel electrophoresis [ 28 ], and single-stranded conformational polymorphism [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed.

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Section: Introductionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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“…Nucleic acid tests Ribosomal RNA gene sequences, and sequencing of the internal transcribed spacer (ITS) region, a procedure widely used for the identification of other mould species, is of limited value for identifying and bar-coding Fusarium species due to the presence of duplicated divergent alleles in this region. For this reason, molecular identification of clinical isolates relies on three loci; TEF-1a (translation elongation factor-1a) (García-Ruiz et al, 2015;Zarrin et al, 2016;Muhammed et al, 2018), RPB1 (the largest subunit of RNA polymerase), and RPB2 (the second largest subunit of RNA polymerase) (Al-Hatmi et al, 2016). Based on these loci, DNA sequence databases have been constructed, and can be interrogated at FUSARIUM-ID (http://isolate.fusariumdb.org/guide.php) and at CBS-KNAW (http://www.westerdijkinstitute.nl/fusarium/), for the identification of environmental and clinical isolates.…”

Section: Detection Of Fusarium Diseases Radiology Histology and Culturementioning

confidence: 99%

Detection of the ‘Big Five’ mold killers of humans: Aspergillus, Fusarium, Lomentospora, Scedosporium and Mucormycetes

Thornton

2020

Advances in Applied Microbiology

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Fungi are an important but frequently overlooked cause of morbidity and mortality in humans. Life-threatening fungal infections mainly occur in immunocompromised patients, and are typically caused by environmental opportunists that take advantage of a weakened immune system. The filamentous fungus Aspergillus fumigatus is the most important and well-documented mould pathogen of humans, causing a number of complex respiratory diseases, including invasive pulmonary aspergillosis, an often fatal disease in patients with acute leukemia or in immunosuppressed bone marrow or solid organ transplant recipients. However, non-Aspergillus moulds are increasingly reported as agents of disseminated diseases, with Fusarium, Scedosporium, Lomentospora and mucormycete species now firmly established as pathogens of immunosuppressed and immunocompetent individuals. Despite well-documented risk factors for invasive fungal diseases, and increased awareness of the risk factors for life-threatening infections, the number of deaths attributable to moulds is likely to be severely underestimated driven, to a large extent, by the lack of readily accessible, cheap, and accurate tests that allow detection and differentiation of infecting species. Early diagnosis is critical to patient survival but, unlike Aspergillus diseases, where a number of CE-marked or FDA-approved biomarker tests are now available for clinical diagnosis, similar tests for fusariosis, scedosporiosis and mucormycosis remain experimental, with detection reliant on insensitive and slow culture of pathogens from invasive bronchoalveolar lavage fluid, tissue biopsy, or from blood. This review examines the ecology, epidemiology, and contemporary methods of detection of these mould pathogens, and the obstacles to diagnostic test development and translation of novel biomarkers to the clinical setting.

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“…Among the most common PCR based techniques used for identification of Fusarium spp. are Random Amplified Polymorphic DNA (RAPD) (Singha et al, 2016), Restriction Fragment Length Polymorphism (RFLP) (Zarrin et al, 2016), Internal Transcript Spacer (ITS) (Ghaffar et al, 2016), Intergenic Space (IGS) (Peltomaa et al, 2016), Elongation Factor (TEF-1-α) (Arif et al, 2012), tubulin ( β-Tub) (Wang et al, 2014), and Inter-Simple Sequence Repeat (ISSR) (Moncrief et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

IDENTIFICATION AND CHARACTERIZATION OF Fusarium spp. FROM MUSKMELON IN NORTHWEST MEXICO

Rivas-García¹,

Montiel²,

Amador³

et al. 2018

BIOTECNIA

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Muskmelon (Cucumis melo L.) is a primary crop of Mexico. Nevertheless, the fruit has a high susceptibility to postharvest fungal diseases. Fusarium species are one of the main causes of diseases that limit production of muskmelon. The objective of this study was to characterize and identify by taxonomic keys and molecular markers species of Fusarium related to rot of muskmelon var. Reticulatus in Northwest Mexico. To identify the causative agent, fruits were collectedm from cultivated fields. The isolated fungi were inoculated on muskmelon to determine its pathogenicity. Morphological analyses as well as molecular techniques confirmed that the pathogen was the fungus Fusarium proliferatum.

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Development of a polymerase chain reaction-restriction fragment length polymorphism method for identification of the Fusarium genus using the transcription elongation factor-1α gene

Cited by 5 publications

References 16 publications

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Detection of the ‘Big Five’ mold killers of humans: Aspergillus, Fusarium, Lomentospora, Scedosporium and Mucormycetes

IDENTIFICATION AND CHARACTERIZATION OF Fusarium spp. FROM MUSKMELON IN NORTHWEST MEXICO

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