Development of a paper-based electrochemical immunosensor using an antibody-single walled carbon nanotubes bio-conjugate modified electrode for label-free detection of foodborne pathogens

Bhardwaj, Jyoti; Devarakonda, Sivaranjani; Kumar, Suveen; Jang, Jaesung

doi:10.1016/j.snb.2017.06.108

Cited by 187 publications

(100 citation statements)

References 52 publications

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“…One of the key advantages of electrochemical sensing technology is its sensitivity and hence detection limits as low as one or very few culture forming units (CFU) have been achieved . However, it has to be questioned if detecting infections at such low bacterial counts will truly be beneficial.…”

Section: Discussionmentioning

confidence: 99%

“…The integration of nanomaterials in biosensors has provided significant improvements in terms of selectivity and analysis time of biosensing methods . Considering the type of nanomaterial for sensor applications is crucial, as most of these materials are rather costly or require lengthy synthesis procedures.…”

Section: Discussionmentioning

confidence: 99%

“…Current research in nanotechnology is growing, and thus further development of smart nanomaterials with useful functions at an efficient cost level is expected for the future. In this context, the presented report has discussed examples for the recent development of paper or plastic‐based materials, which often also offer a facile procedure of handling …”

Section: Discussionmentioning

confidence: 99%

“…Paper‐based sensors have several advantages such as biocompatibility, easiness to pattern, flexibility, lightness and low in costs . In recent years, paper‐based electrochemical biosensors have attracted increasing attention for the detection of bacteria . In general, patterning of papers can be achieved by different methods: wax printing, inkjet printing, screen printing, photolithography, stamping and cutting …”

Section: Literature Reviewmentioning

confidence: 99%

“…In recent literature, Bhardwaj et al. demonstrated a paper‐based electrochemical immunosensor for the label‐free detection of S. aureus , using antibodies, covalently conjugated to SWCNTs, The authors used wax printing method to create hydrophobic barriers on a Whatman paper. Interestingly, after the conjugation of SWCNTs to the paper, the covalent linking of antibodies, specific to S. aureus to SWCNTs resulted in a fabrication process of approximately 2 h, minimizing the damage of the hydrophobic patterns during fabrication and increasing the stability of the sensor for over a month.…”

Section: Literature Reviewmentioning

confidence: 99%

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Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

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Bacterial infections represent one of the leading causes of mortality worldwide, nevertheless the design and development of rapid, cost-efficient and reliable detection methods for pathogens remains challenging. In recent years, electrochemical sensing methods have gained increasing attention for the detection of pathogenic bacteria, due to their increasingly competitive sensitivity. However, combining sensitivity with cost efficiency, high selectivity and a facile working procedure in a portable device is difficult. The presented review provides a summary of biosensing strategies for bacteria, published since 2015, by covering significant achievements towards custom-designed portable point-of-care devices. Herein, the direct chemical recognition of bacteria via enzyme activity or secretion products, as well as their detection at various electrode surfaces and materials, such as nanomaterials, indium tin oxide or paper-based immunosensors, is discussed. Furthermore, newly established hyphenated sensing principles, incorporated into lab-on-a-chip and microfluidic devices, are presented and remaining technical challenges and limitations are considered.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Literature Reviewmentioning

confidence: 99%

Section: Literature Reviewmentioning

confidence: 99%

See 3 more Smart Citations

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

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Nanosensors

Benjamin¹,

Ribeiro²,

Thirumavalavan³

et al. 2022

Bioresource Technology

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Flexible Biosensors for Food Pathogen Detection

Biswas,

Islam,

Jia

et al. 2024

Adv Elect Materials

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Food contamination poses a significant threat to public health, the economy, and human health worldwide, occurring at any stage of the food supply chain, from farm to fork. Efficient and effective real‐time monitoring methods for the early identification and rapid detection of pathogen contamination are critical to preventing possible food safety issues. In the past decade, flexible electrochemical biosensors have rapidly expanded in the detection of foodborne pathogens, owing to their ability to function well at biological interfaces that may be soft, intrinsically curvy, irregular, or deformable. The most important features of flexible sensors are their flexibility, multifunctionality, low cost, and good compatibility with solution processing methods. These properties enable flexible sensors to be used in various applications, including smart packaging. This review focuses on the materials, structures, and fabrication methods of flexible biosensors, as well as their applications in food safety monitoring. It examines recent advances in electrochemical immunosensors and aptasensors for pathogen detection, with a focus on their key components and their application in food analysis, while critically examining their current trends, particularly in conjunction with nanomaterials such as carbon nanotubes, graphene, gold nanoparticles, Indium tin oxide, etc. Finally, current issues and prospects for the future are discussed.

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Development of a paper-based electrochemical immunosensor using an antibody-single walled carbon nanotubes bio-conjugate modified electrode for label-free detection of foodborne pathogens

Cited by 187 publications

References 52 publications

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Nanosensors

Flexible Biosensors for Food Pathogen Detection

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