Development of a pair of differential H/D isotope-coded derivatization reagents d0/d3-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography–tandem mass spectrometry

Sun, Zhi‐Wei; Wang, Xiaoxiang; Cai, Yiping; Fu, Jiang; You, Jinmao

doi:10.1016/j.talanta.2013.11.064

Cited by 25 publications

(20 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this case, MPIA/d 3 -MPIA has been developed, which can be economically and simply prepared with low-cost d 0 /d 3 -iodomethane (CH 3 I/CD 3 I). Moreover, d 0 /d 3 -MPIA, as com-pared with DNPH, exhibits higher sensitivity with mild derivatization conditions for the quantification of aliphatic aldehydes in aquatic products [112].…”

Section: Absolute Quantificationmentioning

confidence: 99%

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

2021

View full text Add to dashboard Cite

Carbonyl-containing metabolites widely exist in biological samples and have important physiological functions. Thus, accurate and sensitive quantitative analysis of carbonyl-containing metabolites is crucial to provide insight into metabolic pathways as well as disease mechanisms. Although reversed phase liquid chromatography electrospray ionization mass spectrometry (RPLC-ESI-MS) is widely used due to the powerful separation capability of RPLC and high specificity and sensitivity of MS, but it is often challenging to directly analyze carbonyl-containing metabolites using RPLC-ESI-MS due to the poor ionization efficiency of neutral carbonyl groups in ESI. Modification of carbonyl-containing metabolites by a chemical derivatization strategy can overcome the obstacle of sensitivity; however, it is insufficient to achieve accurate quantification due to instrument drift and matrix effects. The emergence of stable isotope-coded derivatization (ICD) provides a good solution to the problems encountered above. Thus, LC-MS methods that utilize ICD have been applied in metabolomics including quantitative targeted analysis and untargeted profiling analysis. In addition, ICD makes multiplex or multichannel submetabolome analysis possible, which not only reduces instrument running time but also avoids the variation of MS response. In this review, representative derivatization reagents and typical applications in absolute quantification and submetabolome profiling are discussed to highlight the superiority of the ICD strategy for detection of carbonyl-containing metabolites.

show abstract

Section: Absolute Quantificationmentioning

confidence: 99%

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

2021

View full text Add to dashboard Cite

show abstract

“…2 H 0 / 2 H 3 ‐MPIA was synthesized by mixing 2‐(4‐nitro‐phenyl)‐1 H ‐phenanthro[9,10‐ d ]imidazole with either CH 3 I or C 2 H 3 I. This derivatization technique is characterized by the simplicity of the synthesis of the ICD reagents, economic nature, high sensitivity, and its ability to work in very complex matrices (Sun, Wang, Cai, Fu, & You, ). Thus, the 2 H 0 / 2 H 3 ‐MPIA pair is a good candidate for the analysis of aldehydes in biological fluids.…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 99%

“…2 H 0 / 2 H 3 -MPIA was synthesized by mixing 2-(4-nitro-phenyl)-1H-phenanthro [9,10-d]imidazole with either CH 3 I or C 2 H 3 I. This derivatization technique is characterized by the simplicity of the synthesis of the ICD reagents, economic nature, high sensitivity, and its ability to work in very complex matrices (Sun, Wang, Cai, Fu, & You, 2014 The isotope-coded parts of the reagents are red colored, whereas the reactive group toward the analytes are blue colored. APC, 4-[2-(trimethylammonio)ethoxy]benzenaminium halide; BDHT, 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine; GP, Girard's reagent P; HIQB, 2-(2-hydrazinyl-2-oxoethyl)isoquinolin-2-ium bromide; HMP, 2-hydrazino-1-methylpyridine; ICD, isotope-coded derivatization; MPIA, 4-(1-methyl-1H-phenanthro [9,10-d]…”

Section: Non-hydrazine Based Reagentsmentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

View full text Add to dashboard Cite

Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

show abstract

“…As a conventional regent, originally developed for UV detection, DNP hydrazine enhances the ESI response of analytes but is less efficient in generating a particular product ion by CID. Therefore, numerous derivatization reagents were developed over the past 2 decades aimed to increase selectivity and sensitivity of the LC‐MS analysis; however, they were mainly utilized on selected group of carbonyl compounds and/or sample sources (drinking water, beverages, biological saples, etc). Due to rapid expansion of derivatization‐based LC‐MS studies and application in different fields ranging from protein analysis, metabolite analysis, environmental monitoring to pharmaceutical analysis, and food‐safety control, there is a constant demand for new, sensitive, and selective reagents for the derivatization of carbonyl compounds .…”

Section: Introductionmentioning

confidence: 99%

Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds

Brkljačić

Jerić

2018

J Mass Spectrom

View full text Add to dashboard Cite

Chemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by liquid chromatography-tandem mass spectrometry analysis. The main requirements regarding the good derivatization reagent are fast, selective, and quantitative reaction with carbonyl compounds and efficient fragmentation upon collision-induced dissociation. We have prepared glutamic acid-related hydrazine reagent and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The collision-induced dissociation spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure-specific fragment ions were selected for the development of sensitive multiple reaction monitoring method.

show abstract

Development of a pair of differential H/D isotope-coded derivatization reagents d0/d3-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography–tandem mass spectrometry

Cited by 25 publications

References 37 publications

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

Glutamic acid‐related hydrazine reagent for the derivatization of carbonyl compounds

Contact Info

Product

Resources

About